Morphological and Chemical Comparative Analysis of The Human and Bovine Dentin-Adhesive Layer

Author(s)

Silva Soares, Luis Eduardo ^{[1, 2]} ; do Esprito Santo, Ana Maria ^[3]

Affiliation

^[1] Univ Vale Paraiba, UNIVAP, Dept Dent Mat & Operat Dent, Sch Dent, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba, UNIVAP, Lab Biomed Vibrat Spect, Res & Dev Inst, IP&D, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Dept Ciencias Exatas & Terra, BR-09972270 Sao Paulo - Brazil

• Document type: Journal article
• Source: Microscopy and Microanalysis; v. 21, n. 1, p. 204-213, FEB 2015.
• Web of Science Citations: 5

Abstract

Micro energy-dispersive X-ray fluorescence spectrometry (mu-EDXRF) and scanning electron microscopy (SEM) were used to evaluate dentin treated with an etch and rinse adhesive (ER) and a self-etch adhesive (SE). Ten human molars (H) and ten bovine anterior teeth (B) were prepared (exposure of dentin and divided in the middle) and allocated into two different adhesion strategy groups per substrate (n = 40). mu-EDXRF data and SEM images were obtained before and after treatment. Untreated dentin of both substrates did not differ in terms of Ca (p < 0.1503), P (p < 0.2986) or Ca/P ratio (p < 0.1400). H-SE and B-SE specimens showed reduced P content (p < 0.0001; p < 0.0002), while H-ER and B-ER specimens showed reduced Ca and P content (p < 0.0001; p < 0.0001) when compared with untreated specimens. The Ca/P ratio was significantly higher in H-ER and B-ER specimens than in H-SE and B-SE specimens (p < 0.0001; p < 0.0080). Untreated dentin showed a homogeneous elemental distribution. However, after adhesive treatments, the surface of the dentin showed an irregular demineralization pattern. The resin tags (AU)