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Influence of temporal width pulse of Er:YAG laser in dentin morphology, hybrid layer and bond strength

Grant number: 15/12651-1
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2015
Effective date (End): January 01, 2019
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Patricia Moreira de Freitas Costa e Silva
Grantee:Lívia Tosi Trevelin
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):16/18118-6 - News strategies to prevent adhesive interface degradation, BE.EP.DR


This study aims to evaluate the effect of different irradiation parameters with the Er:YAG laser on the morphology, organic and inorganic composition of the dentin, and the micromorphology and adhesive interface bond strength formed by the irradiated dentin and adhesive system. One hundred and twenty six dentin disks will be irradiated with the Er:YAG laser according to the variation factor (surface treatment), composing 7 experimental groups: G1 without irradiation (control); G2 (200mJ/ 20Hz/50¼s); G3 (200mJ/20Hz/300¼s); G4 (200mJ/20Hz/600¼s); G5 (80mJ/2Hz/50¼s); G6 (80mJ/2Hz/300¼s); G7 (80mJ/2Hz/600¼s). The specimens will be evaluated by different methods, namely: morphological analysis by transmission electron microscopy (TEM) (n=3); evaluation of organic and inorganic constituents of dentin, by micro-Raman spectroscopy (µRS) (n=1); micromorphology evaluation of the adhesive interface by means of confocal microscopy (n=3); and evaluation of the bond strength by microshear test (µSBS) (n=11). The experiments of confocal microscopy and microshear will be mounted using a self-etching adhesive system (Scotchbond Universal, 3M ESPE, USA) together with a composite resin (Z350 XT, 3M ESPE, USA). These specimens will be analyzed after 24 hours and 1 year of artificial saliva storage. So we intend to evaluate the influence of different irradiation parameters on morphological, structural and organic minerals aspects and their interaction with the adhesive system over time. Quantitative data obtained will be analyzed statistically (p <0.05) and qualitative will be described in comparison. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TREVELIN, LIVIA TOSI; ALANIA, YVETTE; MATHEW, MATHEW; PHANSALKAR, RASIKA; CHEN, SHAO-NONG; PAULI, GUIDO F.; BEDRAN-RUSSO, ANA K.. Effect of dentin biomodification delivered by experimental acidic and neutral primers on resin adhesion. Journal of Dentistry, v. 99, . (15/12651-1, 16/18118-6)
TREVELIN, LIVIA TOSI; FERREIRA DA SILVA, BEATRIZ TOGORO; DE FREITAS, PATRICIA MOREIRA; MATOS, ADRIANA BONA. Influence of Er:YAG laser pulse duration on the long-term stability of organic matrix and resin-dentin interface. Lasers in Medical Science, v. 34, n. 7, p. 1391-1399, . (15/12651-1, 15/13571-1)
TREVELIN, LIVIA TOSI; VILLANUEVA, JOSE; ZAMPERINI, CAMILA A.; MATHEW, MATHEW T.; MATOS, ADRIANA BONA; BEDRAN-RUSSO, ANA K.. Investigation of five alpha-hydroxy acids for enamel and dentin etching: Demineralization depth, resin adhesion and dentin enzymatic activity. Dental Materials, v. 35, n. 6, p. 900-908, . (15/12651-1, 16/18118-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TREVELIN, Lívia Tosi. Er:YAG laser protocols influence on dentin morphology, hybrid layer formation and bond strength. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.

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