High precision and selectivity for quantitation of enrofloxacin and ciprofloxacin in five chicken tissues using solid phase extraction and ESI LC-MS/MS for application in monitoring residues

Author(s):Show less -

Gobbo Ferrari, Sarita P. ^[1] ; Bonassa, Karina P. D. ^[2] ; Coelho, Mirela B. ^[3] ; Ferreira, Christina R. ^[3] ; da Costa, Heni Falcao ^[1] ; Jara, Jose Luis P. ^[3] ; Miguel, Maria Carolina V. ^[1] ; Reyes, Felix Guillermo R. ^[2] ; Eberlin, Marcos N. ^[3] ; Nogueira, Guilherme P. ^[1] ; Simas, Rosineide C. ^{[3, 1]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Coll Vet Med, DAPSA, BR-16050680 Aracatuba, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Food Sci, Sch Food Engn, BR-13083862 Campinas, SP - Brazil

^[3] Univ Campinas UNICAMP, Inst Chem, Thomson Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: ANALYTICAL METHODS; v. 7, n. 7, p. 3291-3297, 2015.
• Web of Science Citations: 7

Abstract

Fluoroquinolones (FQs) are synthetic antimicrobials commonly used in intensive poultry farming to treat chronic respiratory disease, colibacillosis, and fowl cholera. As these drugs are also important in treating infections in humans, their use in poultry has been restricted in some countries to avoid the development of antimicrobial-resistant bacteria. In this work, a novel simple and efficient LC-MS/MS method using solid phase extraction (SPE), ESI ionization and multiple reaction mode (MRM) has been developed and validated to determine the two most common FQ residues: enrofloxacin (ENR) and ciprofloxacin (CIP) in chicken target tissues (muscle, liver, kidney, fat and skin) and in plasma. The method is shown to be sensitive, with limits of quantification (LOQ) between 1 ng g(-1) and 5 ng g(-1), recoveries 93-115% and correlation coefficients greater than 0.99. This novel methodology allows faster and simpler sample workflow compared to previously reported methodologies, making it ideal for high-throughput screening and confirmation of both ENR and CIP meeting the international regulation levels. (AU)

• FAPESP's process: 10/51677-2 - Development of new mass spectrometry techniques and their general applications in science: chemistry, biochemistry, material sciences, forensics, medicine, food science, pharmaceutical and veterinary medicine
• Grantee: Marcos Nogueira Eberlin
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 10/15167-0 - Development and Analytical Validation Method of Simultaneous Quantification of Estradiol and Progesterone for LC-MS/MS in Serum/Bovine Blood Plasma
• Grantee: Rosineide Costa Simas
• Support Opportunities: Scholarships in Brazil - Post-Doctoral