ADIPOQ and IL6 variants are associated with a pro-inflammatory status in obeses with cardiometabolic dysfunction

Author(s)

de Oliveira, Raquel ^[1] ; Moraes, Tamiris Invencioni ^[1] ; Cerda, Alvaro ^{[1, 2]} ; Hirata, Mario Hiroyuki ^[1] ; Fajardo, Cristina Moreno ^[1] ; Sousa, Marcela Correia ^[1] ; Dorea, Egidio Lima ^[3] ; Silveira Bernik, Myrcia Martins ^[3] ; Crespo Hirata, Rosario Dominguez ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ La Frontera, BIOREN CEGIN, Ctr Mol Biol & Pharmacogenet, Temuco - Chile

^[3] Univ Sao Paulo, Univ Hosp, BR-05508900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: DIABETOLOGY & METABOLIC SYNDROME; v. 7, APR 11 2015.
• Web of Science Citations: 2

Abstract

Background: Polymorphisms in genes encoding adiponectin (ADIPOQ) and interleukin-6 (IL6) have been associated with adiposity and obese-related phenotypes. This study investigated the relationship of ADIPOQ and IL6 gene polymorphisms with pro-inflammatory and cardiometabolic markers in obese patients. Methods: Anthropometric and body composition parameters were measured in 249 Brazilian subjects (30 to 68 yr). Metabolic and inflammatory markers and adipokines were analyzed in blood samples. ADIPOQ rs2241766 (45 T > G) and IL6 rs1800795 (-174G > C) polymorphisms were analyzed by real-time PCR and PCR-RFLP, respectively. Results: Type 2 diabetes, hypertension, dyslipidemia and increased values of waist circumference, body fat, leptin, fibrinogen, IL-1 beta, hsCRP and TNF alpha were related to obesity (p < 0.05). Multiple linear regression analysis showed a positive correlation between BMI and waist circumference, body fat, leptin, fibrinogen, PAI-1, IL-1 beta, hsCRP and TNF alpha values (p < 0.001) but not with adiponectin. Obese group had altered metabolic status, resistance to leptin and insulin, and atherogenic and pro-inflammatory profiles. ADIPOQ and IL6 variants were not directely related to obesity, leptin resistance or alterations in cardiometabolic markers. Individuals carrying ADIPOQ 45G allele (TG + GG genotype) had higher IL-6, IL-1 beta and TNF alpha levels than TT genotype carriers (p < 0.05). IL6 -174GG genotype was associated with increased IL-1 beta levels (p = 0.033). Conclusion: Obesity is associated with leptin resistance, cardiometabolic alterations and a pro-inflammatory status. Our results are suggestive that ADIPOQ and IL6 polymorphisms contribute to cardiometabolic risk in obese individuals. (AU)

• FAPESP's process: 09/10069-2 - Influence of genetic makers on response to a dietary intervention to reduce body weight
• Grantee: Rosario Dominguez Crespo Hirata
• Support Opportunities: Regular Research Grants