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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia

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Author(s):
Gelaleti, Rafael B. [1] ; Damasceno, Debora C. [1] ; Salvadori, Daisy M. F. [2] ; Marcondes, Joao Paulo C. [2] ; Lima, Paula H. O. [1] ; Morceli, Glilciane [1] ; Calderon, Iracema M. P. [1] ; Rudge, Marilza V. C. [1]
Total Authors: 8
Affiliation:
[1] Unesp Univ Estadual Paulista, Lab Expt Res Gynecol & Obstet, Dept Gynecol & Obstet, Botucatu Med Sch, BR-18618000 Sao Paulo - Brazil
[2] Unesp Univ Estadual Paulista, Dept Pathol, Lab Toxigen & Nutrigen, Botucatu Med Sch, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: DIABETOLOGY & METABOLIC SYNDROME; v. 7, APR 2 2015.
Web of Science Citations: 1
Abstract

Background: Pregnant women with mild gestational hyperglycemia present a high risk for hypertension and obesity, and appear to reproduce the model of metabolic syndrome in pregnancy, including hyperinsulinemia and insulin resistance. Diabetic patients have a higher frequency of the IRS-1 Gly972Arg variant and this polymorphism is directly related to insulin resistance and subsequent hyperglycemia. In diabetes, hyperglycemia and other associated factors generate reactive oxygen species that increase DNA damage. The aims of this study were to evaluate the presence of the IRS-1 Arg972 polymorphism in pregnant women with diabetes or mild gestational hyperglycemia, and in their newborns. Additionally, we evaluated the level of primary DNA damage in lymphocytes of Brazilian pregnant women and the relationship between the amount of genetic damage and presence of the polymorphism. Methods: A based on the oral glucose tolerance test (OGTT) results and on glycemic profiles (GP), as follows: nondiabetic group, mild gestational hyperglycemia (MGH) and diabetic group. Eighty-five newborns were included in the study. Maternal peripheral blood samples and umbilical cord blood samples (5-10 mL) were collected for genotyping by PCR-RFLP and for comet assays. Results: The prevalence of genotype Gly/Arg in pregnant women groups was not statistically significant. In newborns, the frequency of Gly/Arg was significantly higher in the MGH and diabetic groups than in the non-diabetic group. Taken together, groups IIA and IIB (IIA + IIB; diabetes) presented lower amounts of DNA damage than the non-diabetic group (p = 0.064). No significant association was detected between genetic damage and the presence of the Arg972 genotype in pregnant women. Conclusion: The polymorphism was more prevalent in newborns of diabetic and MGH women. We believe that it is necessary to increase the number of subjects to be examined in order to better determine the biological role of the Arg972 polymorphism in these patients. Despite being classified as low-risk, pregnant women with mild gestational hyperglycemia characterize a population of maternal and perinatal adverse outcomes, and that, together with their newborns, require better monitoring by professionals and health services. (AU)

FAPESP's process: 08/06642-6 - Evaluation of pregnant women genotoxicity with positive diagnosis of mild gestational hyperglycaemia
Grantee:Marilza Vieira Cunha Rudge
Support Opportunities: Regular Research Grants
FAPESP's process: 08/06480-6 - Evaluation of genes and proteins of blood and placenta of diabetic pregnant women and with mild gestational hyperglycaemia
Grantee:Marilza Vieira Cunha Rudge
Support Opportunities: Regular Research Grants