Effects of eugenol and dehidrodieugenol isolated from Nectandra leucantha (Laurace...
| Full text | |
| Author(s): Show less - |
da Costa-Silva, Thais Alves
[1]
;
Grecco, Simone S.
[2]
;
de Sousa, Fernanda S.
[3]
;
Lago, Joao Henrique G.
[3]
;
Martins, Euder G. A.
[4]
;
Terrazas, Cesar A.
[5, 6]
;
Varikuti, Sanjay
[6, 5]
;
Owens, Katherine L.
[7]
;
Beverley, Stephen M.
[7]
;
Satoskar, Abhay R.
[5, 6]
;
Tempone, Andre G.
[1]
Total Authors: 11
|
| Affiliation: | [1] Adolfo Lutz Inst, Ctr Parasitol & Mycol, Sao Paulo - Brazil
[2] Fed Univ ABC, Ctr Nat Sci & Humanities, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Sao Paulo - Brazil
[4] Univ Sao Paulo, Dept Bot, Inst Biosci, Sao Paulo - Brazil
[5] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 - USA
[6] Ohio State Univ, Dept Pathol, Columbus, OH 43210 - USA
[7] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 - USA
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Journal of Natural Products; v. 78, n. 4, p. 653-657, APR 2015. |
| Web of Science Citations: | 23 |
| Abstract | |
Three phenylpropanoid dimers (1-3) including two new metabolites were isolated from the extract of the twigs of Nectandra leucantha using antileishmanial bioassay-guided fractionation. The in vitro antiparasitic activity of the isolated compounds against Leishmania donovani parasites and mammalian cytotoxicity and immunomodulatory effects were evaluated. Compounds 1-3 were effective against the intracellular amastigotes within macrophages, with IC50 values of 26.7, 17.8, and 101.9 mu M, respectively. The mammalian cytotoxicity, given by the 50% cytotoxic concentration (CC50), was evaluated against peritoneal macrophages. Compounds 1 and 3 were not toxic up to 290 mu M, whereas compound 2 demonstrated a CC50 value of 111.2 mu M. Compounds 1-3 also suppressed production of disease exacerbatory cytokines IL-6 and IL-10 but had minimal effect on nitric oxide production in L. donovani-infected macrophages, indicating that antileishmanial activity of these compounds is mediated via an NO-independent mechanism. Therefore, these new natural products could represent promising scaffolds for drug design studies for leishmaniasis. (AU) | |
| FAPESP's process: | 13/50318-7 - Natural product-based drug design: novel compounds against Leishmania (L.) infantum |
| Grantee: | André Gustavo Tempone Cardoso |
| Support type: | Regular Research Grants |
| FAPESP's process: | 12/18756-1 - Evaluation of novel alternative therapies with synthetic drugs using in vitro and experimental models of Leishmania (L.) infantum chagasi |
| Grantee: | André Gustavo Tempone Cardoso |
| Support type: | Regular Research Grants |
| FAPESP's process: | 13/07275-5 - In vitro study of cellular immune response against synthetic drugs with antileishmanial activities |
| Grantee: | Thaís Alves da Costa Silva |
| Support type: | Scholarships in Brazil - Post-Doctorate |