Etidronate causes minimal changes in the ability of sodium hypochlorite to dissolve organic matter

Author(s)

Tartari, T. ^[1] ; Guimaraes, B. M. ^[1] ; Amoras, L. S. ^[2] ; Duarte, M. A. H. ^[1] ; Silva e Souza, P. A. R. ^[2] ; Bramante, C. M. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dent Sch Bauru, Dept Endodont, Bauru, SP - Brazil

^[2] Fed Univ Para, Sch Dent, Dept Endodont, BR-66059 Belem, Para - Brazil

• Document type: Journal article
• Source: International Endodontic Journal; v. 48, n. 4, p. 399-404, APR 2015.
• Web of Science Citations: 20

Abstract

AimTo evaluate the effect of individual and combined use of sodium hypochlorite (NaOCl), etidronate (HEDP) and ethylenediaminetetraacetic acid (EDTA) in tissue dissolution. MethodologySixty fragments of bovine muscle tissue were prepared and their weights determined on a precision scale. The samples were then distributed in the following groups (n=10): G1 - saline solution (control); G2 - 17% EDTA; G3 - 18% HEDP; G4 - 2.5% NaOCl; G5 - mixture of 5% NaOCl+17% EDTA; and G6 - mixture of 5% NaOCl+18% HEDP. The specimens in each group were immersed in the solutions for 5, 10 and 15min and reweighted at each time period. Analysis of variance (anova) and Tukey's multiple-comparison tests (<0.05) were applied to identify the intragroup and intergroup differences. ResultsG1, G2, G3 and G5 did not dissolve the organic matter. G4 and G6 significantly reduced the weights of specimens at all periods. Amongst the groups, the difference in ability to dissolve organic matter was greater and significant in the following order G4=G6>G5=G3=G2=G1 after 5min of immersion and G4>G6>G5=G3=G2=G1 after 10 and 15min of immersion. ConclusionThe only solution capable of dissolving organic matter was NaOCl. In the mixtures analysed, this ability was arrested by EDTA; however, it was minimally affected by the HEDP, proving that this combination, if used during the biomechanical preparation, is able to dissolve of organic matter. (AU)

• FAPESP's process: 13/19789-3 - Effects of different irrigation regimes in physico-chemical properties of dentin and consequences of changesin the adhesion of microorganisms and AH Plus sealer
• Grantee: Talita Tartari
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/06738-9 - Evaluation of materials, instruments and protocols of irrigation used in Endodontic treatment, by means of scanning electron microscopy and micro analysis of x-ray.
• Grantee: Marco Antonio Hungaro Duarte
• Support Opportunities: Regular Research Grants