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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of human and microbial DNA content in subgingival plaque samples collected by paper points or curette

Full text
Author(s):
Perez-Chaparro, P. J. [1] ; Duarte, P. M. [1] ; Pannuti, C. M. [2] ; Figueiredo, L. C. [1] ; Mestnik, M. J. [1] ; Goncalves, C. P. S. [1] ; Faveri, M. [1] ; Feres, M. [1]
Total Authors: 8
Affiliation:
[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, BR-07023070 Guarulhos, SP - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Microbiological Methods; v. 111, p. 19-20, APR 2015.
Web of Science Citations: 2
Abstract

Host DNA may adversely affect metagenomic studies focusing on the prokaryotic microbiota. This study compared the levels of host DNA in subgingival plaque collected by paper points and curette, using quantitative PCR. Lower proportions of host DNA and higher proportions of bacterial DNA were recovered from samples collected with curettes. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/20915-0 - Metagenomic analysis of periodontal subgingival microbiota in subjects with and without periodontal disease
Grantee:Paula Juliana Perez Chaparro
Support Opportunities: Scholarships in Brazil - Post-Doctoral