Pinealectomy interferes with the circadian clock genes expression in white adipose tissue

Author(s):Show less -

Mendes de Farias, Talita da Silva ^[1] ; de Oliveira, Ariclecio Cunha ^[1] ; Andreotti, Sandra ^[1] ; do Amaral, Fernanda Gaspar ^[2] ; Chimin, Patricia ^[1] ; Alves de Proenca, Andre Ricardo ^[1] ; Leonardo Torres Leal, Francisco ^[1] ; Laurato Sertie, Rogerio Antonio ^[1] ; Campana, Amanda Baron ^[1] ; Lopes, Andressa Bolsoni ^[1] ; de Souza, Arnaldo Henrique ^[1] ; Cipolla-Neto, Jose ^[1] ; Lima, Fabio Bessa ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Physiol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Pineal Research; v. 58, n. 3, p. 251-261, APR 2015.
• Web of Science Citations: 26

Abstract

Melatonin, the main hormone produced by the pineal gland, is secreted in a circadian manner (24-hr period), and its oscillation influences several circadian biological rhythms, such as the regulation of clock genes expression (chronobiotic effect) and the modulation of several endocrine functions in peripheral tissues. Assuming that the circadian synchronization of clock genes can play a role in the regulation of energy metabolism and it is influenced by melatonin, our study was designed to assess possible alterations as a consequence of melatonin absence on the circadian expression of clock genes in the epididymal adipose tissue of male Wistar rats and the possible metabolic repercussions to this tissue. Our data show that pinealectomy indeed has impacts on molecular events: it abolishes the daily pattern of the expression of Clock, Per2, and Cry1 clock genes and Ppar expression, significantly increases the amplitude of daily expression of Rev-erb, and affects the pattern of and impairs adipokine production, leading to a decrease in leptin levels. However, regarding some metabolic aspects of adipocyte functions, such as its ability to synthesize triacylglycerols from glucose along 24hr, was not compromised by pinealectomy, although the daily profile of the lipogenic enzymes expression (ATP-citrate lyase, malic enzyme, fatty acid synthase, and glucose-6-phosphate dehydrogenase) was abolished in pinealectomized animals. (AU)

• FAPESP's process: 10/20719-1 - Melatonin, the circadian expression of clock genes in adipose tissue of adult male rats and the control of lipolitic activity.
• Grantee: Talita da Silva Mendes de Farias Carvalho
• Support Opportunities: Scholarships in Brazil - Doctorate