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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of Metabolic Changes in Plant Pathosystems by Imprint Imaging DESI-MS

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Author(s):
Tata, Alessandra [1, 2] ; Perez, Consuelo J. [1] ; Hamid, Tanam S. [1] ; Bayfield, Mark A. [3] ; Ifa, Demian R. [1]
Total Authors: 5
Affiliation:
[1] York Univ, Dept Chem, Ctr Res Mass Spectrometry, Toronto, ON M3J 2R7 - Canada
[2] Univ Campinas UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, Sao Paulo - Brazil
[3] York Univ, Dept Biol, Toronto, ON M3J 2R7 - Canada
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY; v. 26, n. 4, p. 641-648, APR 2015.
Web of Science Citations: 19
Abstract

The response of plants to microbial pathogens is based on the production of secondary metabolites. The complexity of plant-pathogen interactions makes their understanding a challenging task for metabolomic studies requiring powerful analytical approaches. In this paper, the ability of ambient mass spectrometry to provide a snapshot of plant metabolic response to pathogen invasion was tested. The fluctuations of glycoalkaloids present in sprouted potatoes infected by the phytopathogen Pythium ultimum were monitored by imprint imaging desorption electrospray ionization mass spectrometry (DESI-MS). After 8 d from the inoculation, a decrease of the relative abundance of potato glycoalkaloids alpha-solanine (m/z 706) and alpha-chaconine (m/z 722) was observed, whereas the relative intensity of solanidine (m/z 398), solasodenone (m/z 412), solanaviol (m/z 430), solasodiene (m/z 396), solaspiralidine (m/z 428), gamma-solanine/gamma-chaconine (m/z 560) , beta-solanine (m/z 706), and beta-chaconine (m/z 722) increased. The progression of the disease, expressed by the development of brown necrotic lesions on the potato, led to the further decrease of all the glycoalkaloid metabolites. Therefore, the applicability of imprint imaging DESI-MS in studying the plant metabolic changes in a simple pathosystem was demonstrated with minimal sample preparation. (AU)

FAPESP's process: 13/15575-9 - Screening and mapping of fungal quorum sensing by ambient mass spectrometry: chemical profiling and localization of molecules involved in networking biocontrol among fungal agents and different phytopathogens
Grantee:Alessandra Tata
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor