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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Eccentric Exercise Leads to Glial Activation but not Apoptosis in Mice Spinal Cords

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Author(s):
Pereira, B. C. [1] ; Lucas, G. [2] ; da Rocha, A. L. [1] ; Pauli, J. R. [3] ; Ropelle, E. R. [4] ; Cintra, D. [4] ; de Souza, C. T. [5] ; Bueno, C. R. [6] ; da Silva, A. S. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, Lab Pain Neurobiol, Ribeirao Preto - Brazil
[3] State Univ Campinas UNICAMP, Fac Sci Appl, Sport Sci Course, Campinas, SP - Brazil
[4] State Univ Campinas UNICAMP, Fac Sci Appl, Sport Sci Course, Limeira - Brazil
[5] Univ Southern Santa Catarina, Exercise Biochem & Physiol Lab, Postgrad Program Hlth Sci, Hlth Sci Unit, Criciuma - Brazil
[6] Univ Sao Paulo, Human Genome Res Ctr, Sao Paulo, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF SPORTS MEDICINE; v. 36, n. 5, p. 378-385, MAY 2015.
Web of Science Citations: 17
Abstract

The aim of this investigation was to evaluate the effects of 3 overtraining (OT) protocols on the glial activation and apoptosis in the spinal cords of mice. Rodents were divided into control (C; sedentary mice), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR). The incremental load test, ambulation test, exhaustive test and functional behavioural assessment were used as performance evaluation parameters. 36h after the exhaustive test, the dorsal and ventral parts of the lumbar spinal cord (L4-L6) were dissected for subsequent protein analysis by immunoblotting. The OT protocols led to similar responses of some performance parameters. The ventral glial fibrillary acidic protein (GFAP) protein levels were diminished in the OTR/up and OTR compared to CT and OTR/down groups. The ventral ionized calcium binding adaptor molecule 1 (Iba-1), and the dorsal GFAP and Iba-1 protein levels were increased in the OTR/down compared to the other groups. The ratio between the cleaved capase-3/caspase-3 and cleaved caspase-9/caspase-9 measured in the spinal cord were not sensitive to the OT protocols. In summary, the OTR/down activated the glial cells in the motor (i.e. Iba-1) and sensory (i.e. GFAP and Iba-1) neurons without leading to apoptosis. (AU)

FAPESP's process: 13/20591-3 - Responses of proteins from the inflammatory, insulinic and hypertrophic molecular pathways to nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in skeletal muscle of mice
Grantee:Adelino Sanchez Ramos da Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 13/19985-7 - Effects of overtraining induced by exercise in treadmill without inclination, uphill and downhill on the content and activation of proteins of insulin signaling pathway and inflamation in mice
Grantee:Bruno Cesar Pereira
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 13/22737-5 - Effects of nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in content and activation of the mTOR pathway proteins in skeletal muscle of mice
Grantee:Alisson Luiz da Rocha
Support Opportunities: Scholarships in Brazil - Scientific Initiation