| Full text | |
| Author(s): |
Pereira, B. C.
[1]
;
Lucas, G.
[2]
;
da Rocha, A. L.
[1]
;
Pauli, J. R.
[3]
;
Ropelle, E. R.
[4]
;
Cintra, D.
[4]
;
de Souza, C. T.
[5]
;
Bueno, C. R.
[6]
;
da Silva, A. S.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, Lab Pain Neurobiol, Ribeirao Preto - Brazil
[3] State Univ Campinas UNICAMP, Fac Sci Appl, Sport Sci Course, Campinas, SP - Brazil
[4] State Univ Campinas UNICAMP, Fac Sci Appl, Sport Sci Course, Limeira - Brazil
[5] Univ Southern Santa Catarina, Exercise Biochem & Physiol Lab, Postgrad Program Hlth Sci, Hlth Sci Unit, Criciuma - Brazil
[6] Univ Sao Paulo, Human Genome Res Ctr, Sao Paulo, SP - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF SPORTS MEDICINE; v. 36, n. 5, p. 378-385, MAY 2015. |
| Web of Science Citations: | 17 |
| Abstract | |
The aim of this investigation was to evaluate the effects of 3 overtraining (OT) protocols on the glial activation and apoptosis in the spinal cords of mice. Rodents were divided into control (C; sedentary mice), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR). The incremental load test, ambulation test, exhaustive test and functional behavioural assessment were used as performance evaluation parameters. 36h after the exhaustive test, the dorsal and ventral parts of the lumbar spinal cord (L4-L6) were dissected for subsequent protein analysis by immunoblotting. The OT protocols led to similar responses of some performance parameters. The ventral glial fibrillary acidic protein (GFAP) protein levels were diminished in the OTR/up and OTR compared to CT and OTR/down groups. The ventral ionized calcium binding adaptor molecule 1 (Iba-1), and the dorsal GFAP and Iba-1 protein levels were increased in the OTR/down compared to the other groups. The ratio between the cleaved capase-3/caspase-3 and cleaved caspase-9/caspase-9 measured in the spinal cord were not sensitive to the OT protocols. In summary, the OTR/down activated the glial cells in the motor (i.e. Iba-1) and sensory (i.e. GFAP and Iba-1) neurons without leading to apoptosis. (AU) | |
| FAPESP's process: | 13/20591-3 - Responses of proteins from the inflammatory, insulinic and hypertrophic molecular pathways to nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in skeletal muscle of mice |
| Grantee: | Adelino Sanchez Ramos da Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/19985-7 - Effects of overtraining induced by exercise in treadmill without inclination, uphill and downhill on the content and activation of proteins of insulin signaling pathway and inflamation in mice |
| Grantee: | Bruno Cesar Pereira |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/22737-5 - Effects of nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in content and activation of the mTOR pathway proteins in skeletal muscle of mice |
| Grantee: | Alisson Luiz da Rocha |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |