Pereira, B. C.
da Rocha, A. L.
Pauli, J. R.
Ropelle, E. R.
de Souza, C. T.
Bueno, C. R.
da Silva, A. S.
Total Authors: 9
 Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto - Brazil
 Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, Lab Pain Neurobiol, Ribeirao Preto - Brazil
 State Univ Campinas UNICAMP, Fac Sci Appl, Sport Sci Course, Campinas, SP - Brazil
 State Univ Campinas UNICAMP, Fac Sci Appl, Sport Sci Course, Limeira - Brazil
 Univ Southern Santa Catarina, Exercise Biochem & Physiol Lab, Postgrad Program Hlth Sci, Hlth Sci Unit, Criciuma - Brazil
 Univ Sao Paulo, Human Genome Res Ctr, Sao Paulo, SP - Brazil
Total Affiliations: 6
INTERNATIONAL JOURNAL OF SPORTS MEDICINE;
Web of Science Citations:
The aim of this investigation was to evaluate the effects of 3 overtraining (OT) protocols on the glial activation and apoptosis in the spinal cords of mice. Rodents were divided into control (C; sedentary mice), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR). The incremental load test, ambulation test, exhaustive test and functional behavioural assessment were used as performance evaluation parameters. 36h after the exhaustive test, the dorsal and ventral parts of the lumbar spinal cord (L4-L6) were dissected for subsequent protein analysis by immunoblotting. The OT protocols led to similar responses of some performance parameters. The ventral glial fibrillary acidic protein (GFAP) protein levels were diminished in the OTR/up and OTR compared to CT and OTR/down groups. The ventral ionized calcium binding adaptor molecule 1 (Iba-1), and the dorsal GFAP and Iba-1 protein levels were increased in the OTR/down compared to the other groups. The ratio between the cleaved capase-3/caspase-3 and cleaved caspase-9/caspase-9 measured in the spinal cord were not sensitive to the OT protocols. In summary, the OTR/down activated the glial cells in the motor (i.e. Iba-1) and sensory (i.e. GFAP and Iba-1) neurons without leading to apoptosis. (AU)