Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Significant differences in integration sites of Moloney murine leukemia virus/Moloney murine sarcoma virus retroviral vector carrying recombinant coagulation factor IX in two human cell lines

Full text
Author(s):
Castilho-Fernandes, Andrielle [1, 2, 3] ; Fontes, Aparecida Maria [4] ; Abraham, Kuruvilla Joseph [5] ; Correa de Freitas, Marcela Cristina [1, 2, 3] ; da Rosa, Nathalia Gonsales [1, 2, 6] ; Picanco-Castro, Virginia [1, 2] ; de Sousa Russo-Carbolante, Elisa Maria [1, 2, 7] ; Covas, Dimas Tadeu [1, 2, 3]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Ctr Cell Therapy, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Reg Blood Ctr, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Dept Clin Med, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto - Brazil
[5] Univ Sao Paulo, Dept Pediat, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil
[6] Univ Sao Paulo, Dept Pharmaceut Sci, Fac Pharmaceut Sci Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil
[7] Univ Sao Paulo, Dept Clin Toxicol & Food Sci Anal, Fac Pharmaceut Sci Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil
Total Affiliations: 7
Document type: Journal article
Source: Biotechnology Letters; v. 37, n. 5, p. 991-1001, MAY 2015.
Web of Science Citations: 0
Abstract

Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive elements, fragile sites and target genes. In HepG2, rhFIX was had an increased preference for chromosomes 6 and 17; the median distance to the nearest CpG islands was 15,240 base pairs and 37 % of the integrations occurred in RefSeq genes. In Hek293, rhFIX had an increased preference for chromosome 5; the median distance to the nearest CpG islands was 209,100 base pairs and 74 % of the integrations occurred in RefSeq genes. The integrations in both cell lines were distant from the TSS. The integration patterns associated with this vector are different in each cell line. (AU)

FAPESP's process: 98/14247-6 - Center for Research on Cell-Based Therapy
Grantee:Marco Antonio Zago
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 08/57877-3 - National Institute of Science and Technology in Cell Therapy
Grantee:Roberto Passetto Falcão
Support type: Research Projects - Thematic Grants