Different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: Microhardness and polarized light miscroscopy analyses

Author(s)

De Campos, Priscila Hernandez ^[1] ; Sanabe, Mariane Emi ^[2] ; Rodrigues, Jonas Almeida ^[3] ; Duarte, Danilo Antonio ^[1] ; Botti Rodrigues Santos, Maria Teresa ^[4] ; Guare, Renata Oliveira ^[1] ; Duque, Cristiane ^[5] ; Lussi, Adrian ^[6] ; Diniz, Michele Baffi ^[1]

Affiliation

^[1] Cruzeiro Sul Univ UNICSUL, Dept Pediat Dent, BR-01506000 Sao Paulo, SP - Brazil

^[2] Camilo Castelo Branco Univ UNICATELO, Dept Pediat Dent, BR-04204002 Sao Paulo, SP - Brazil

^[3] Rio Grande Sul Fed Univ UFRGS, Sch Dent, Dept Pediat Dent, BR-90035003 Porto Alegre, RS - Brazil

^[4] Cruzeiro Sul Univ UNICSUL, Patients Special Needs, BR-01506000 Sao Paulo, SP - Brazil

^[5] Sao Paulo State Univ UNESP, Aracatuba Sch Dent, Dept Pediat Dent, BR-16015050 Aracatuba, SP - Brazil

^[6] Univ Bern UNIBE, Sch Dent Med, Dept Prevent Restorat & Pediat Dent, CH-3010 Bern - Switzerland

• Document type: Journal article
• Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 78, n. 6, p. 444-451, JUN 2015.
• Web of Science Citations: 6

Abstract

The aim of this study was to compare different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions by microhardness and polarized light microscopy analyses. One hundred blocks of bovine enamel were randomly divided into four groups (n=25) according to the bacterial model for caries induction: (A) Streptococcus mutans, (B) S. mutans and Lactobacillus acidophilus, (C) S. mutans and L. casei, and (D) S. mutans, L. acidophilus, and L. casei. Within each group, the blocks were randomly divided into five subgroups according to the duration of the period of caries induction (4-20 days). The enamel blocks were immersed in cariogenic solution containing the microorganisms, which was changed every 48 h. Groups C and D presented lower surface hardness values (SMH) and higher area of hardness loss (S) after the cariogenic challenge than groups A and B (P<0.05). As regards lesion depth, under polarized light microscopy, group A presented significantly lower values, and groups C and D the highest values. Group B showed a higher value than group A (P<0.05). Groups A and B exhibited subsurface caries lesions after all treatment durations, while groups C and D presented erosion-type lesions with surface softening. The model using S. mutans, whether or not it was associated with L. acidophilus, was less aggressive and may be used for the induction of non-cavitated enamel caries-like lesions. The optimal period for inducing caries-like lesions was 8 days. Microsc. Res. Tech. 78:444-451, 2015. (c) 2015 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 10/06855-0 - Performance of fluorescence-based methods in monitoring non cavitated caries-like lesions on smooth surfaces using a bacterial cariogenic model in vitro
• Grantee: Michele Baffi Diniz
• Support Opportunities: Regular Research Grants