Advanced search
Start date
Betweenand

Evaluation of in vitro cariogenic potential of bifidobacteria species.

Grant number: 14/02072-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2014
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Cristiane Duque
Grantee:Vanessa Rodrigues dos Santos
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Streptococcus mutans is not the only species associated with caries lesions. Other potentially acidogenic and aciduric bacterial species, such as bifidobacteria, have been detected in various oral sites, including the initial carious lesions or cavities. Many of these bifidobacteria, called probiotics, are used in the food industry and consequently are widely consumed by children. The objectives of this study will be compare the ability to produce and tolerate acids, to form biofilm and to induce in vitro initial carious lesions by species of bifidobacteria compared to bacterial species already recognized in the context of dental caries. The following bacterial species will be included in this study: Bifidobacterium lactis, Bifidobacterium longum , Bifidobacterium animalis, Bifidobacterium dentium, Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus casei and Actinomyces israelli. In order to evaluate the cariogenic potential of these bacterial species, acidogenicity tests will be carried out by checking the final pH of the cultures after exposure to high glucose concentration, aciduric tests will be conducted to measure bacterial growth after exposure to acidic pH and in vitro biofilm formation will quantify biofilm biomass in an ELISA reader. Finally , tests for induction of initial caries lesions in bovine teeth will be conducted to check the thickness of the demineralized enamel using polarized light microscopy. Tests of biofilm and caries lesion induction will be performed with all strains combined or not with Streptococcus mutans. The data will be statistically analyzed, considering pd0,05.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VANESSA RODRIGUES DOS SANTOS; REMBERTO MARCELO ARGANDOÑA VALDEZ; MARCELLE DANELON; JOSÉ ANTONIO SANTOS SOUZA; KARINA SAMPAIO CAIAFFA; ALBERTO CARLOS BOTAZZO DELBEM; CRISTIANE DUQUE. Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization. Brazilian Oral Research, v. 35, p. -, 2021.
ARGANDONA VALDEZ, REMBERTO MARCELO; DUQUE, CRISTIANE; CAIAFFA, KARINA SAMPAIO; DOS SANTOS, VANESSA RODRIGUES; DE AGUIAR LOESCH, MARIA LUIZA; COLOMBO, NATALIA HELENA; ARTHUR, RODRIGO ALEX; NEGRINI, THAIS DE CASSIA; GOMES BORIOLLO, MARCELO FABIANO; BOTAZZO DELBEM, ALBERTO CARLOS. Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries. BMC ORAL HEALTH, v. 17, JUL 14 2017. Web of Science Citations: 6.
ARGANDONA VALDEZ, REMBERTO MARCELO; DOS SANTOS, VANESSA RODRIGUES; CAIAFFA, KARINA SAMPAIO; DANELON, MARCELLE; ARTHUR, RODRIGO ALEX; NEGRINI, THAIS DE CASSIA; BOTAZZO DELBEM, ALBERTO CARLOS; DUQUE, CRISTIANE. Comparative in vitro investigation of the cariogenic potential of bifidobacteria. ARCHIVES OF ORAL BIOLOGY, v. 71, p. 97-103, NOV 2016. Web of Science Citations: 6.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.