Noninvasive monitoring of photodynamic therapy on skin neoplastic lesions using the optical attenuation coefficient measured by optical coherence tomography

Author(s)

Goulart, Viviane P. ^[1] ; dos Santos, Moises O. ^[2] ; Latrive, Anne ^[1] ; Freitas, Anderson Z. ^[1] ; Correa, Luciana ^[3] ; Zezell, Denise M. ^[1]

Affiliation

^[1] IPEN CNEN SP, CLA, Lab Biofoton, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Estado Amazonas, Escola Super Tecnol, BR-69050020 Manaus, AM - Brazil

^[3] Univ Sao Paulo, Fac Odontol, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Journal of Biomedical Optics; v. 20, n. 5 MAY 2015.
• Web of Science Citations: 3

Abstract

Photodynamic therapy (PDT) has become a promising alternative for treatment of skin lesions such as squamous cell carcinoma. We propose a method to monitor the effects of PDT in a noninvasive way by using the optical attenuation coefficient (OAC) calculated from optical coherence tomography (OCT) images. We conducted a study on mice with chemically induced neoplastic lesions and performed PDT on these lesions using homemade photosensitizers. The response of neoplastic lesions to therapy was monitored using, at the same time, macroscopic clinical visualization, histopathological analysis, OCT imaging, and OCT-based attenuation coefficient measurement. Results with all four modalities demonstrated a positive response to treatment. The attenuation coefficient was found to be 1.4 higher in skin lesions than in healthy tissue and it decreased after therapy. This study shows that the OAC is a potential tool to noninvasively assess the evolution of skin neoplastic lesions with time after treatment. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) (AU)

• FAPESP's process: 05/51689-2 - Optics and Photonics Research Center at UNICAMP
• Grantee: Hugo Luis Fragnito
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC