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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers

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Author(s):
Mori, Enio [1, 2] ; Lara, Maria do Carmo C. S. H. [3] ; Cunha, Elenice M. S. [3] ; Villalobos, Eliana M. C. [3] ; Mori, Claudia M. C. [1] ; Soares, Rodrigo M. [4] ; Brandao, Paulo E. [4] ; Fernandes, Wilson R. [5] ; Richtzenhain, Leonardo J. [4]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Patol, Fac Med Vet & Ciencia Anim, Sao Paulo, SP - Brazil
[2] Inst Pasteur, BR-01418000 Sao Paulo, SP - Brazil
[3] Inst Biol, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Fac Med Vet & Ciencia Anim, Dept Clin Med, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Brazilian Journal of Microbiology; v. 46, n. 2, p. 565-U255, APR-JUN 2015.
Web of Science Citations: 2
Abstract

Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1 archival strains (A3/97, A4/72, A9/92) and three clinical samples (Iso07/05, Iso11/06 and Iso33/06) suggested that among Brazilian EHV-1 strains, the amplified region of the gD gene sequence is highly conserved. Additionally, the analysis of ICP4 gene showed high nucleotide and amino acid identities when compared with genotype P strains, suggesting that the EHV-1 Brazilian strains belonged to the same group. All the EHV-1 Brazilian strains were classified as non-neuropathogenic variants (N752) based on the ORF30 analysis. These findings indicate a high conservation of the gD-, ICP4- and ORF30-encoding sequences. Different pathotypes of the EHV-1 strain might share identical genes with no specific markers, and tissue tropism is not completely dependent on the gD envelope, immediate-early ICP4 and DNA polymerase proteins. (AU)