| Full text | |
| Author(s): |
Cagnini, Didier Q.
[1]
;
Cunha, Paulo H. J.
[2]
;
Pantoja, Jose C. F.
[3]
;
Badial, Peres R.
[1]
;
de Oliveira-Filho, Jose Paes
[1]
;
Araujo-Junior, Joao P.
[1]
;
Alfieri, Amauri A.
[4]
;
Borges, Alexandre S.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Paulista Unesp, Fac Med Vet & Zootecnia, Dept Clin Vet, BR-18618970 Botucatu, SP - Brazil
[2] Univ Fed Goias, Escola Vet Zootecnia, Dept Med Vet, BR-74001970 Goiania, Go - Brazil
[3] FMVZ Unesp, Dept Higiene Vet & Saude Publ, Botucatu, SP - Brazil
[4] Univ Estadual Londrina, Dept Vet Prevent Med, Ctr Ciencias Agr, Lab Virol Anim, BR-86057970 Londrina, PR - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 35, n. 4, p. 337-343, APR 2015. |
| Web of Science Citations: | 3 |
| Abstract | |
Bovine meningoencephalitis caused by BHV-5, a double-stranded DNA enveloped virus that belongs to the family Herpesviridae and subfamily Alphaherpesvirinae, is an important differential diagnosis of central nervous diseases. The aim of this study was to describe the histological changes in the central nervous system of calves experimentally infected with BHV-5 and compare these changes with the PCR and IHC results. Formalin-fixed paraffin-embedded central nervous system samples from calves previously inoculated with BHV-5 were microscopically evaluated and tested using IHC and PCR. All the animals presented with nonsuppurative meningoencephalitis. From 18 evaluated areas of each calf, 32.41% and 35.19% were positive by IHC and PCR, respectively. The telencephalon presented more accentuated lesions and positive areas in the PCR than other encephalic areas and was the best sampling area for diagnostic purposes. Positive areas in the IHC and PCR were more injured than IHC and PCR negative areas. The animal with neurological signs showed more PCR- and IHC-positive areas than the other animals. (AU) | |
| FAPESP's process: | 08/07697-9 - BHV-5 diagnostic in parafin embeded bovine brain tissue using polymerase chain reaction assay, immunohistochemical and histopathological examination |
| Grantee: | Didier Quevedo Cagnini |
| Support Opportunities: | Scholarships in Brazil - Master |