Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Insulin Modulates Liver Function in a Type I Diabetes Rat Model

Full text
Author(s):
Nolasco, Eduardo L. [1] ; Zanoni, Fernando L. [2] ; Nunes, Fernanda P. B. [1] ; Ferreira, Sabrina S. [1] ; Freitas, Luiza A. [1] ; Silva, Mariana C. F. [1] ; Martins, Joilson O. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo FCF USP, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Lab Immunoendocrinol, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Med, Heart Inst InCor, LIM 11, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY; v. 36, n. 4, p. 1467-1479, 2015.
Web of Science Citations: 7
Abstract

Background/Aims: Several studies have been performed to unravel the association between diabetes and increased susceptibility to infection. This study aimed to investigate the effect of insulin on the local environment after cecal ligation and puncture (CLP) in rats. Methods: Diabetic (alloxan, 42 mg/kg i.v., 10 days) and non-diabetic (control) male Wistar rats were subjected to a two-puncture CLP procedure and 6 h later, the following analyses were performed: (a) total and differential cell counts in peritoneal lavage (PeL) and bronchoalveolar lavage (BAL) fluids; (b) quantification of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, IL-6, IL-10 and cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-2 in the PeL and BAL fluids by enzyme-linked immunosorbent assay (ELISA); (c) total leukocyte count using a veterinary hematology analyzer and differential leukocyte counts on stained slides; (d) biochemical parameters (urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) by colorimetric analyses); and (e) lung, kidney, and liver morphological analyses (hematoxylin and eosin staining). Results: Relative to controls, non-diabetic and diabetic CLP rats exhibited an increased in the concentration of IL-1 beta, IL-6, IL-10, CINC-1, and CINC-2 and total and neutrophil in the PeL fluid. Treatment of these animals with neutral protamine Hagedorn insulin (NPH, 1IU and 4IU, respectively, s.c.), 2 hours before CLP procedure, induced an increase on these cells in the PeL fluid but it did not change cytokine levels. The levels of ALT, AST, ALP, and urea were higher in diabetic CLP rats than in non-diabetic CLP rats. ALP levels were higher in diabetic sham rats than in non-diabetic sham rats. Treatment of diabetic rats with insulin completely restored ALT, AST, and ALP levels. Conclusion: These results together suggest that insulin attenuates liver dysfunction during early two-puncture CLP-induced peritoneal inflammation in diabetic rats. Copyright (C) 2015 S. Karger AG, Basel (AU)

FAPESP's process: 14/05214-1 - Investigating the role of insulin in different infections in diabetic and healthy animals
Grantee:Joilson de Oliveira Martins
Support Opportunities: Regular Research Grants
FAPESP's process: 10/02272-0 - Effect of insulin on lung inflammation in animal with sepsis, innate immunit,activation of insulin gene (BGK) and insulin receptors (IR)-A and IR-B
Grantee:Joilson de Oliveira Martins
Support Opportunities: Research Grants - Young Investigators Grants