Acute Exercise Decreases Tribbles Homolog 3 Protein Levels in the Hypothalamus of Obese Rats

Author(s):Show less -

Rodrigues, Barbara De Almeira ^{[1, 2]} ; Souza Pauli, Luciana Santos ^{[1, 2]} ; De Souza, Claudio Teodoro ^[3] ; Ramos Da Silva, Adelino Sanchez ^[4] ; Correa Cintra, Dennys Esper ^{[1, 2]} ; Marinho, Rodolfo ^[5] ; De Moura, Leandro Pereira ^[5] ; Chiarreotto Ropelle, Eloize Cristina ^{[1, 2]} ; Botezelli, Jose Diego ^{[1, 2]} ; Ropelle, Eduardo Rochete ^{[1, 2]} ; Pauli, Jose Rodrigo ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Ctr Res Sport Sci, Sch Appl Sci, Lab Mol Biol Exercise, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Ctr Res Sport Sci, Sch Appl Sci, Ctr Biol Assays, Sao Paulo - Brazil

^[3] Univ Extremo Sul Catarinense, Hlth Sci Unit, Lab Exercise Biochem & Physiol, Criciuma, SC - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Sao Paulo - Brazil

^[5] Sao Paulo Univ State, Sao Paulo - Brazil

• Document type: Journal article
• Source: MEDICINE AND SCIENCE IN SPORTS AND EXERCISE; v. 47, n. 8, p. 1613-1623, AUG 2015.
• Web of Science Citations: 11

Abstract

Purpose This study aims to evaluate the effects of acute exercise on tribbles homolog 3 (TRB3) protein levels and on the interaction between TRB3 and Akt proteins in the hypothalamus of obese rats. In addition, we evaluated the relationship between TRB3 and endoplasmic reticulum (ER) stress and verified whether an acute exercise session influences them. Methods In the first part of the study, the rats were divided into three groups: control (lean), fed standard rodent chow; DIO, fed a high-fat diet; and DIO-EXE, fed a high-fat diet and submitted to a swimming acute exercise protocol. In the second part of the study, we used three other groups: control (lean) group receiving an intracerebroventricular (i.c.v.) infusion of vehicle, lean group receiving an i.c.v. infusion of thapsigargin, and lean group receiving an i.c.v. infusion of thapsigargin and performing an acute exercise session. Four hours after the exercise session, food intake was measured, and the hypothalamus was dissected and separated for subsequent protein analysis by immunoblotting and real-time polymerase chain reaction. Results The acute exercise session reduced TRB3 protein levels, disrupted the interaction between TRB3 and Akt proteins, increased the phosphorylation of Foxo1, and restored the anorexigenic effects of insulin on the hypothalamus of DIO rats. Interestingly, the suppressive effects of acute exercise on TRB3 protein levels may be related, at least in part, to decreased ER stress (evaluated though pancreatic ER kinase phosphorylation and C/EBP homologous protein levels) in the hypothalamus. Conclusion Exercise-mediated reduction of hypothalamic TRB3 protein levels may be associated with reduction of ER stress. These data provide a new mechanism by which an acute exercise session improves insulin sensitivity in the hypothalamus and restores food intake control in obesity. (AU)

• FAPESP's process: 13/21491-2 - Role of physical exercise in regulation of rock protein and the effects on insulin signaling in skeletal muscle and liver in obese mice
• Grantee: José Rodrigo Pauli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/25512-4 - Role of physical exercise in the regulation of MKP-3 protein in the hypothalamus of obese rats: effects on insulin signaling.
• Grantee: Barbara de Almeida Rodrigues
• Support Opportunities: Scholarships in Brazil - Doctorate