Effects of Estrogen Deficiency and/or Caffeine Intake on Alveolar Zone Loss, Density, and Healing: A Study in Rats

Author(s)

Bezerra, Joyce Pinho ^[1] ; de Siqueira, Ariane ^[1] ; Pires, Amanda Goncalves ^[1] ; Marques, Marcelo Rocha ^[2] ; Duarte, Poliana Mendes ^[1] ; Bastos, Marta Ferreira ^[1]

Affiliation

^[1] Univ Guarulhos, Dent Res Div, Dept Periodont, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Sch Dent Piracicaba, Div Histol, Dept Morphol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Periodontology; v. 84, n. 6, p. 839-849, JUN 2013.
• Web of Science Citations: 11

Abstract

Background: The aim of this study is to evaluate the effects of caffeine and/or estrogen deficiency on ligature-induced bone loss (BL), trabecular bone area (TBA), and postextraction bone healing (BH). Methods: Rats were assigned into one of the following groups (15 each): 1) control = non-ingestion of caffeine/sham surgery; 2) caffeine = ingestion of caffeine/sham surgery); 3) ovariectomized (OVX) = non-ingestion of caffeine/ovariectomy; or 4) caffeine/OVX = ingestion of caffeine/ovariectomy. The rats were under caffeine administration for 65 days and/or estrogen deficiency for 51 days. On day 21 after ovariectomy, one first mandibular molar received a ligature and the contralateral tooth was not ligated. The first maxillary molars were extracted 8 days before sacrifice. BL, TBA, the positive cells for tartrate-resistant acid phosphatase (TRAP), receptor activator of nuclear factor-kappa B ligand (RANKL), and osteoprotegerin (OPG) were analyzed in the furcation area of mandibular molars. Histometric BH and gene expression of bone morphogenetic protein (BMP)-2, BMP-7, osteopontin, and bone sialoprotein were evaluated in alveolar sockets. Results: The caffeine group presented the greatest BL and the OVX group the highest number of TRAP-positive (TRAP) cells around ligated teeth (P < 0.05). The control group presented higher TBA and BH than the other groups (P < 0.05). All test groups presented higher RANKL/OPG(+) cells than the control group around ligated/unligated teeth. The OVX and caffeine/OVX groups presented a greater number of TRAP(+) cells around unligated teeth than the control group (P < 0.05). There were no differences among groups for gene expression (P>0.05). Conclusions: Caffeine increased BL in ligated teeth. Caffeine and/or estrogen deficiency decreased TBA in the unligated teeth and reduced BH after tooth extraction. (AU)

• FAPESP's process: 09/09446-6 - Impact of chronic intake of caffeine associated or not with osteoporosis on the ligature induced-bone loss, bone density and healing in rats
• Grantee: Marta Ferreira Bastos
• Support Opportunities: Regular Research Grants