The effect of low-level laser therapy (LLLT) applied prior to muscle injury

Author(s)

Ribeiro, Beatriz Guimaraes ^[1] ; Alves, Agnelo Neves ^[1] ; Dias dos Santos, Lucas Andreo ^[2] ; Santos Fernandes, Kristianne Porta ^{[2, 1]} ; Cantero, Tatiane Matarazzo ^[3] ; Gomes, Mariana Teixeira ^[2] ; Franca, Cristiane Miranda ^[2] ; Teixeira da Silva, Daniela de Fatima ^[2] ; Bussadori, Sandra Kalil ^{[2, 1]} ; Mesquita-Ferrari, Raquel Agnelli ^{[2, 1]}

Affiliation

^[1] Univ Nove de Julho UNINOVE, Rehabil Sci, BR-01504001 Sao Paulo, SP - Brazil

^[2] Univ Nove de Julho UNINOVE, Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo, SP - Brazil

^[3] Univ Nove de Julho UNINOVE, BR-01504001 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Surgery and Medicine; v. 47, n. 7, p. 571-578, SEP 2015.
• Web of Science Citations: 10

Abstract

AimTo evaluate the effect of LLLT (780nm; 10J/cm(2), 40mW, 3.2J) prior to injury on the morphological analysis, collagen deposition, and activity of matrix metalloproteinase-2 (MMP-2). MethodsWistar rats were divided into groups: control; sham; only LLLT; only muscle injury and LLLT+injury. The rats were euthanized at 1, 3, and 7 days following cryoinjury to muscle that was removed for analysis. ResultsLLLT applied prior to muscle injury led to a reduction in myonecrosis and inflammatory cells, an increase of blood vessels and immature muscle fibers. An increase in MMP-2 activity and a decrease in collagen deposition were also found, with a better collagen organization and distribution. ConclusionLLLT applied immediately prior to injury had positive effects during the muscle regeneration process. Therefore, this resource may have considerable therapeutic value, especially for athletes who practice sports in which there is a constant risk of muscle injury. Lasers Surg. Med. 47:571-578, 2015. (c) 2015 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 14/12381-1 - Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury
• Grantee: Raquel Agnelli Mesquita Ferrari
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/11461-6 - Effects of early treatment with low level laser therapy on the muscle skeletal repair
• Grantee: Beatriz Guimarães Ribeiro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/25198-5 - Effects of early treatment with low level laser therapy on matrix metalloprotease 2 activity during skeletal muscle repair
• Grantee: Tatiane Matarazzo Cantero
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/21540-3 - Comparative effect of low-level laser therapy and low intensity ultrasound on myofiber phenotypes, oxidative stress and proteins during muscle repair
• Grantee: Agnelo Neves Alves
• Support Opportunities: Scholarships in Brazil - Doctorate