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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of the bacteriocin produced by cheese isolate Lactobacillus paraplantarum FT259 and its potential influence on Listeria monocytogenes biofilm formation

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Author(s):
Winkelstroeter, Lizziane Kretli [1] ; Tulini, Fabricio Luiz [1] ; De Martinis, Elaine C. P. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo FCFRP USP, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: LWT-FOOD SCIENCE AND TECHNOLOGY; v. 64, n. 2, p. 586-592, DEC 2015.
Web of Science Citations: 19
Abstract

Lactic acid bacteria (LAB) are known by their technological properties, such as the inhibition of spoilage and pathogenic bacteria. The aims of this study were: (i) to evaluate the influence of Lactobacillus paraplantarum FT259 on biofilm formation by Listeria monocytogenes by using culture method and fluorescence in situ hybridization (FISH) and, (ii) to partially characterize the bacteriocin produced by this strain isolated from Brazilian semi-hard cheese by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). We also performed polymerase chain reactions with primers for plantaricin NC8, plantaricin S and plantaricin W structural genes followed by DNA sequencing to possibly identify the bacteriocin produced. Results of microscopy assays demonstrated the complex and heterogenous structure of the biofilm formed by co-culture of both strains. SDS-PAGE analysis indicated that the partially purified bacteriocin presented a molecular mass around 3900 Da and, PCR-DNA sequencing detected the plantaricin NC8 gene. To our knowledge, this is the first report on the inhibitory effect of an L. paraplantarum strain on L monocytogenes biofilms, as well as the formation of biofilm by this LAB species. In addition, we have demonstrated L paraplantarum FT259 harbored the gene for plantaricin NC8 production, which may be responsible by the antimicrobial activity observed. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 09/51141-8 - Gene expression analysis, biofilm formation and adherence to Caco-2 cells by Listeria monocytogenes in different conditions found in food
Grantee:Elaine Cristina Pereira de Martinis
Support Opportunities: Regular Research Grants
FAPESP's process: 11/11983-0 - Isolation of lactic acid bacteria from milk and cheese with potential for food biopreservation and utilization for increasing whey digestibility
Grantee:Fabricio Luiz Tulini
Support Opportunities: Scholarships in Brazil - Doctorate