System for evaluation of oxidative stress on in-vitro-produced bovine embryos

Author(s)

de Assis, Patricia Monken ^[1] ; Castro, Leticia Signori ^[1] ; Perez Siqueira, Adriano Felipe ^[1] ; Delgado, Juliana de Carvalho ^[1] ; dos Santos Hamilton, Thais Rose ^[1] ; Goissis, Marcelo Demarchi ^[2] ; Mendes, Camilla Mota ^{[1, 2]} ; Nichi, Marcilio ^[3] ; Visintin, Jose Antonio ^[2] ; Ortiz D'Avila Assumpacao, Mayra Elena ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med & Anim Sci, Lab Spermatozoa Biol, BR-05508 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med & Anim Sci, Lab Vitro Fertilizat Cloning & Anim Transgenesis, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med & Anim Sci, Lab Androl, BR-05508 Sao Paulo - Brazil

• Document type: Journal article
• Source: Reproductive BioMedicine Online; v. 31, n. 4, p. 577-580, OCT 2015.
• Web of Science Citations: 8

Abstract

This study proposed a quantitative evaluation of oxidative status (OS) in bovine embryos. Sixteen-cell stage embryos, cultured under 5% O-2, were treated with oxidative stress inducer menadione (0, 1, 2.5 and 5 mu mol/l) for 24 h. Blastocyst rate (BLR) was recorded and expanded blastocysts were stained with CellROX (R) Green (CRG; OS evaluation) and evaluated under epifluorescence microscopy (ratio of pixel/blastomere). A significant effect of menadione was observed for BLR (P = 0.0039), number of blastomeres/embryo (P < 0.0001) and OS (P < 0.001). Strong negative correlations were found between BLR and the number of blastomeres with OS evaluation, demonstrating the efficacy of this analysis to evaluate OS levels of IVF bovine embryos. (C) 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 11/21529-4 - Evaluation of melatonin antioxidant properties upon fresh and cryopreserved in vitro produced bovine embryos
• Grantee: Mayra Elena Ortiz D' Avila Assumpcao
• Support Opportunities: Regular Research Grants