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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cyto- and genotoxic effects of metallic nanoparticles in untransformed human fibroblast

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Franchi, Leonardo P. [1] ; Manshian, Bella B. [2] ; de Souza, Tiago A. J. [1] ; Soenen, Stefaan J. [2] ; Matsubara, Elaine Y. [3] ; Rosolen, J. Mauricio [3] ; Takahashi, Catarina S. [1, 4]
Total Authors: 7
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Katholieke Univ Leuven, Fac Med, MoSAIC Biomed MRI Unit, Leuven - Belgium
[3] Univ Sao Paulo, Dept Chem, Fac Philosophy Sci & Letters Ribeirao Preto, Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, Dept Biol, Fac Philosophy Sci & Letters Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 29, n. 7, p. 1319-1331, OCT 2015.
Web of Science Citations: 26

Metallic nanoparticles such as silver (Ag), cerium dioxide (CeO2) and titanium dioxide (TiO2) are produced at a large scale and included in many consumer products. It is well known that most metallic NPs are toxic to humans which raise concerns about these engineered particles. Various studies have already been published on the subject, however, almost all of these studies have been conducted in cancer or transformed cell lines. In this work we performed a comparative evaluation of these metallic NPs on normal untransformed human fibroblasts (GM07492) detecting cyto- and geno-toxic responses after exposure to these NPs. Our results showed that all three metallic NPs were able to cross the plasma membrane and were mainly found in endocytic vesicles. The Ag and TiO2 NPs affected mitochondrial enzymatic activity (XTT), increased DNA fragmentation, oxidative damage (Comet assay) and induced cell death mainly by the apoptotic pathway. Ag NPs increased GADD45 alpha transcript levels and the phosphorylation of proteins gamma H2AX. Transient genotoxicity was also observed from exposure to CeO2 NPs while TiO2 NPs showed no increase in DNA damage at sub-cytotoxic concentrations. In comparison, Ag NPs were found to be the most cyto-genotoxic NPs to fibroblasts. Thus, these results support the use of normal fibroblast as a more informative tool to detect the mechanisms of action induced by metallic NPs. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 10/10863-8 - Cytotoxic and genotoxic effects of nanoparticles in cell lines in vitro.
Grantee:Leonardo Pereira Franchi
Support type: Scholarships in Brazil - Doctorate