Aerobic exercise training enhances the in vivo cholesterol trafficking from macrophages to the liver independently of changes in the expression of genes involved in lipid flux in macrophages and aorta

Author(s):Show less -

Pinto, Paula Ramos ^[1] ; Ferraretto Moura Rocco, Debora Dias ^[1] ; Okuda, Ligia Shimabukuro ^[1] ; Machado-Lima, Adriana ^[1] ; Castilho, Gabriela ^[1] ; da Silva, Karolline Santana ^[1] ; Gomes, Diego Juvenal ^[1] ; Pinto, Raphael de Souza ^[1] ; Iborra, Rodrigo Tallada ^[1] ; Ferreira, Guilherme da Silva ^[1] ; Nakandakare, Edna Regina ^[1] ; Machado, Ubiratan Fabres ^[2] ; Cardillo Correa-Giannella, Maria Lucia ^[3] ; Catanozi, Sergio ^[1] ; Passarelli, Marisa ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Med, Lipids Lab LIM 10, BR-01246000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-01246000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Lab Endocrinol & Cellular Metab LIM 25, BR-01246000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: LIPIDS IN HEALTH AND DISEASE; v. 14, SEP 16 2015.
• Web of Science Citations: 10

Abstract

Background: Regular exercise prevents and regresses atherosclerosis by improving lipid metabolism and antioxidant defenses. Exercise ameliorates the reverse cholesterol transport (RCT), an antiatherogenic system that drives cholesterol from arterial macrophages to the liver for excretion into bile and feces. In this study we analyzed the role of aerobic exercise on the in vivo RCT and expression of genes and proteins involved in lipid flux and inflammation in peritoneal macrophages, aortic arch and liver from wild type mice. Methods: Twelve-week-old male mice were divided into sedentary and trained groups. Exercise training was performed in a treadmill (15 m/min, 30 min/day, 5 days/week). Plasma lipids were determined by enzymatic methods and lipoprotein profile by fast protein liquid chromatography. After intraperitoneal injection of J774-macrophages the RCT was assessed by measuring the recovery of H-3-cholesterol in plasma, feces and liver. The expression of liver receptors was determined by immunoblot, macrophages and aortic mRNAs by qRT-PCR. C-14-cholesterol efflux mediated by apo A-I and HDL2 and the uptake of H-3-cholesteryl oleoyl ether (H-3-COE)-acetylated-LDL were determined in macrophages isolated from sedentary and trained animals 48 h after the last exercise session. Results: Body weight, plasma lipids, lipoprotein profile, glucose and blood pressure were not modified by exercise training. A greater amount of H-3-cholesterol was recovered in plasma (24 h and 48 h) and liver (48 h) from trained animals in comparison to sedentary. No difference was found in H-3-cholesterol excreted in feces between trained and sedentary mice. The hepatic expression of scavenger receptor class B type I (SR-BI) and LDL receptor (B-E) was enhanced by exercise. We observed 2.8 and 1.7 fold rise, respectively, in LXR and Cyp7a mRNA in the liver of trained as compared to sedentary mice. Macrophage and aortic expression of genes involved in lipid efflux was not systematically changed by physical exercise. In agreement, C-14-cholestrol efflux and uptake of H-3-COE-acetylated-LDL by macrophages was similar between sedentary and trained animals. Conclusion: Aerobic exercise in vivo accelerates the traffic of cholesterol from macrophages to the liver contributing to prevention and regression of atherosclerosis, independently of changes in macrophage and aorta gene expression. (AU)

• FAPESP's process: 12/19112-0 - Involvement of the RAGE/AGE axis in macrophage lipid accumulation induced by human advanced glycated albumin - contribution of glycemic control in diabetes mellitus.
• Grantee: Adriana Machado Saldiba de Lima
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/04631-0 - Advanced glycation end-products and renin-angiotensin system: impact on the development of atherosclerosis in dislipidemic mice
• Grantee: Diego Juvenal Gomes
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/12088-7 - Glycemic control and the removal of macrophage cholesterol by ABCA-1: role of modified albumin by advanced glycation
• Grantee: Rodrigo Tallada Iborra
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/15153-1 - Aerobic exercise training in wild type and CETP transgenic mice does not affect cellular cholesterol removal and expression of genes involved in lipid flux in macrophages and aortic arch
• Grantee: Paula Ramos Pinto
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 12/04831-1 - New players in glycemic control and chronic complications of Diabetes mellitus: preventive and therapeutic perspectives
• Grantee: Ubiratan Fabres Machado
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/18724-2 - Advanced glycated albumin and insulin resistance in rats: focus on periepididimal adipose tissue and N-acetylcysteine actions
• Grantee: Karolline Santana da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/02854-7 - Anti-inflammatory role of apolipoprotein A-IV in diabetes mellitus and its impact on macrophage reverse cholesterol transport: influence of advanced glycation
• Grantee: Ligia Shimabukuro Okuda
• Support Opportunities: Scholarships in Brazil - Post-Doctoral