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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Monoamine oxidase B layer-by-layer film fabrication and characterization toward dopamine detection

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Miyazaki, Celina Massumi [1] ; Pereira, Tamyris Paschoal [1] ; Tavares Mascagni, Daniela Branco [2] ; de Moraes, Marli Leite [3] ; Ferreira, Marystela [1]
Total Authors: 5
[1] Univ Fed Sao Carlos, UFSCar, CCTS, Sao Paulo - Brazil
[2] Univ Estadual Sao Paulo UNESP, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, UNIFESP, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Materials Science & Engineering C-Materials for Biological Applications; v. 58, p. 310-315, JAN 1 2016.
Web of Science Citations: 12

In this work nanostructured film composites of the monoamine oxidase B (MAO-B) enzyme, free or encapsulated in liposomes, were fabricated by the layer-by-layer (LbL) self-assembly technique, employing polyethylene imine (PEI) as polycation. Initially, the MAO-B enzyme was incorporated into liposomes in order to preserve its enzymatic structure ensuring their activity and catalytic stability. The LbL film growth was monitored by surface plasmon resonance (SPR) by gold resonance angle shift analysis after each bilayer deposition. Subsequently, the films were applied as amperometric biosensors for dopamine detection using Prussian Blue (PB) as the electron mediator. The biosensor fabricated by MAO-B incorporated into liposomes composed of DPPG:POPG in the ratio (1:4) (w/w) showed the best performance with a sensitivity of 0.86 (mu A cm(-2))/(mmol L-1) and a detection limit of 033 mmol L-1. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/15093-7 - Study of nanostructured systems by surface plasmon resonance (SPR): applications in biosensing
Grantee:Celina Massumi Miyazaki
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/17519-1 - Immobilization of metal nanoparticules in ultrathin films aiming biosensing
Grantee:Marystela Ferreira
Support type: Regular Research Grants