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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of nutritional parameters in bovine milk by Raman spectroscopy with least squares modeling

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Author(s):
Silveira, Jr., Landulfo [1] ; Martins Motta, Edlene do Carmo [1] ; Zangaro, Renato Amaro [1] ; Tavares Pacheco, Marcos Tadeu [1] ; de Lima, Carlos Jose [1] ; Moreira, Livia Helena [1]
Total Authors: 6
Affiliation:
[1] Univ Camilo Castelo Branco UNICASTELO, Inst Biomed Engn, Sao Jose - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Instrumentation Science & Technology; v. 44, n. 1, p. 85-97, JAN 2 2016.
Web of Science Citations: 6
Abstract

The macronutrient constitution of bovine milk was investigated by Raman spectroscopy involving 830nm excitation at 300 mW from 600-1800cm(-1) with a probe. Bovine milk was analyzed for total proteins, casein, total fat, and lactose by standard assays. The concentrations of these components were estimated by correlating these assays with the fitting coefficients based on least-squares fitting of lactose, casein, triolein, and stearic acid and milk spectra to prepare calibration curves. The model was employed for the analysis of milk from local markets. The spectra of milk were dominated by bands of proteins, lipids, and carbohydrates. There was a strong correlation between the concentrations of total proteins and casein (r=0.75 and 0.73, respectively), and very strong correlation for total fat and lactose (r=0.93 and 0.91, respectively) with the standard errors of prediction of 0.49, 0.38, 0.61, and 0.27mg/dL, respectively. The milk contained lower concentrations of lactose and protein than in the nutritional facts table. The amount of saturated fat was close to the nutritional value with more unsaturated fat. A single Raman spectrum was employed to characterize the composition of milk and may have application for rapid quality control. (AU)

FAPESP's process: 09/01788-5 - Dispersive Raman spectroscopy using optical fibers "Raman probe" applied to the diagnosis of neoplasia in the skin and prostate
Grantee:Landulfo Silveira Junior
Support Opportunities: Regular Research Grants