A DNA tool for the identification of heavily exploited Atlantic billfishes

Author(s)

Domingues, Rodrigo R. ^[1] ; Okuda, Gabriel ^[2] ; Bernard, Andrea M. ^[3] ; Amorim, Alberto F. ^[4] ; De Biasi, Juliana ^[2] ; Hilsdorf, Alexandre W. S. ^[2]

Affiliation

^[1] Univ Estadual Paulista UNESP, Inst Biociencias Rio Claro, BR-11350690 Rio Claro, SP - Brazil

^[2] Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, BR-08701970 Mogi Das Cruzes - Brazil

^[3] Nova SE Univ, Oceanog Ctr, Guy Harvey Res Inst, Dania, FL 33004 - USA

^[4] Inst Pesca, Agencia Paulista Tecnol Agronegocios, BR-11030906 Santos, SP - Brazil

• Document type: Journal article
• Source: CONSERVATION GENETICS RESOURCES; v. 7, n. 4, p. 807-809, DEC 2015.
• Web of Science Citations: 1

Abstract

Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR-RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes (TaqI and HaeIII) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions. (AU)

• FAPESP's process: 09/54660-6 - DNA mitochondrial sequence analysis for genetic population and molecular identification assessment of economically important pelagic fish species landed in fishery ports of State of São Paulo coast
• Grantee: Alexandre Wagner Silva Hilsdorf
• Support Opportunities: Regular Research Grants