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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of low-level laser therapy on the expression of osteogenic genes during the initial stages of bone healing in rats: a microarray analysis

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Author(s):
Tim, Carla Roberta [1] ; Bossini, Paulo Sergio [2] ; Kido, Hueliton Wilian [1] ; Malavazi, Iran [3] ; von Zeska Kress, Marcia Regina [4] ; Carazzolle, Marcelo Falsarella [5, 6] ; Parizotto, Nivaldo Antonio [1] ; Renn, Ana Claudia [2]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos, Dept Physiotherapy, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biosci, BR-11050240 Santos - Brazil
[3] Univ Fed Sao Carlos, Dept Genet & Evolut, BR-13565905 Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Bromatol, BR-14049900 Ribeirao Preto - Brazil
[5] Univ Estadual Campinas, Dept Genet & Evolut, BR-13083970 Campinas, SP - Brazil
[6] Brazilian Natl Ctr Res Energy & Mat, Brazilian Biosci Natl Lab, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Lasers in Medical Science; v. 30, n. 9, p. 2325-2333, DEC 2015.
Web of Science Citations: 9
Abstract

This study evaluated the morphological changes produced by LLLT on the initial stages of bone healing and also studied the pathways that stimulate the expression of genes related to bone cell proliferation and differentiation. One hundred Wistar rats were divided into control and treated groups. Noncritical size bone defects were surgically created at the upper third of the tibia. Laser irradiation (Ga-Al-As laser 830 nm, 30 mW, 94 s, 2.8 J) was performed for 1, 2, 3, 5, and 7 sessions. Histopathology revealed that treated animals produced increased amount of newly formed bone at the site of the injury. Moreover, microarray analysis evidenced that LLLT produced a significant increase in the expression TGF-beta, BMP, FGF, and RUNX-2 that could stimulate osteoblast proliferation and differentiation, which may be related to improving the deposition of newly formed bone at the site of the injury. Thus, it is possible to conclude that LLLT improves bone healing by producing a significant increase in the expression of osteogenic genes. (AU)

FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
Grantee:Munir Salomao Skaf
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 10/15335-0 - Analysis of gene expression during bone repair defects in tibia irradiated with low intensity laser therapy.
Grantee:Carla Roberta Tim
Support Opportunities: Scholarships in Brazil - Doctorate