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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sex-related differences in lung inflammation after brain death

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Author(s):
Breithaupt-Faloppa, Ana Cristina [1] ; Ferreira, Sueli G. [1] ; Kudo, Guilherme K. [1] ; Armstrong, Jr., Roberto [1] ; Tavares-de-Lima, Wothan [2] ; Ferraz da Silva, Luiz Fernando [3] ; Sannomiya, Paulina [1] ; Moreira, Luiz Felipe P. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Med, Lab Cardiovasc Surg & Circulat Pathophysiol LIM 1, Heart Inst InCor, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF SURGICAL RESEARCH; v. 200, n. 2, p. 714-721, FEB 2016.
Web of Science Citations: 3
Abstract

Background: Donor sex has been suggested to be a factor influencing organ transplantation outcome. Sex hormones possess inflammatory and immune-mediating properties; therefore, immune responses may differ between males and females. Brain death (BD) affects organ function by numerous mechanisms including alterations in hemodynamics, hormonal changes, and increased systemic inflammation. In this study, we investigated sex-dependent differences in the evolution of lung inflammation in a rat model of BD. Materials and methods: BD was induced by a sudden increase in intracranial pressure by rapidly inflating a balloon catheter inserted into the intracranial space. Groups of male, female, and ovariectomized (OVx) female rats were used. Lung vascular permeability, inducible nitric oxide synthase, and intercellular adhesion molecule 1 expression were analyzed 6 h after BD. Serum female sex hormones, vascular endothelial growth factor, and cytokine-induced neutrophil chemoattractant 1 levels were also quantified. Lung sections were analyzed by histology. Results: After 6 h of BD, serum estradiol and progesterone concentrations in female rats were significantly reduced. Lung microvascular permeability was increased in females compared to males. Cytokine-induced neutrophil chemoattractant 1 and vascular endothelial growth factor concentrations were increased in female rats compared to males. Furthermore, female rats showed higher levels of leukocyte infiltration and inducible nitric oxide synthase expression in the lung parenchyma. Conclusions: Our results indicate that the more severe lung inflammation in female animals after BD might be related to acute estradiol reduction. Based on our findings, we believe that, in a future study, a group of female treated with estradiol after BD could indicate a possible therapy for the control of lung inflammation in the female donor. (C) 2016 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 13/20282-0 - Gender influence on the microcirculatory repercussions and the inflammatory process in different organs in a model of brain death in rats
Grantee:Ana Cristina Breithaupt Faloppa
Support Opportunities: Regular Research Grants