Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells

Author(s)

Serpeloni, Juliana Mara ^[1] ; Leal Specian, Ana Flavia ^[2] ; Ribeiro, Diego Luis ^[2] ; Tuttis, Katiuska ^[2] ; Vilegas, Wagner ^[3] ; Martinez-Lopez, Wilner ^[4] ; Dokkedal, Anne Ligia ^[5] ; Saldanha, Luiz Leonardo ^{[6, 5]} ; de Syllos Colus, Ilce Mara ^[2] ; Varanda, Eliana Aparecida ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara - Brazil

^[2] State Univ Londrina UEL, Dept Gen Biol, Ctr Biol Sci, BR-86057970 Londrina - Brazil

^[3] Sao Paulo State Univ UNESP, BR-11350000 Sao Vicente - Brazil

^[4] Inst Invest Biol Clemente Estable, Montevideo 11600 - Uruguay

^[5] Sao Paulo State Univ UNESP, Dept Biol Sci, Fac Sci, BR-17033360 Bauru - Brazil

^[6] Sao Paulo State Univ UNESP, Inst Biosci, Dept Bot, BR-18618970 Botucatu, SP - Brazil

• Document type: Journal article
• Source: Journal of Ethnopharmacology; v. 176, p. 345-355, DEC 24 2015.
• Web of Science Citations: 6

Abstract

Ethnopharmacological relevance: The Brazilian ``Cerrado{''} is an important source of natural products, such as Myrcia bella Cambess (MB, also known as ``mercurinho{''}). MB leaves are popularly used for the treatment of diabetes and gastrointestinal disorders; however, only its hypoglycemic activity has been experimentally described. Aim of the study: Because MB is used to treat gastrointestinal disorders, the present study characterized biological activities of hydroalcoholic MB extract in human normal and tumor gastric cells. Materials and methods: Cytotoxic, antiproliferative, genotoxic and protective effects were evaluated, as well as the effects of the MB extract on gene expression. Results: The MB extract induced cytotoxicity in tumor cells at lower concentrations compared with normal cells as assessed by the MTT assay. Moreover, the MB extract induced necrosis based on acridine orange/ethidium bromide staining. An antiproliferative effect was evidenced through an arrest in the G2/M phase detected by flow cytometry and a decrease in the nuclear division index using the cytokinesis-block micronucleus cytome assay. Cells treated with MB extract combined with doxorubicin (DXR) showed increased NUBDs, which may be related to the gene amplification of CCND1. Antimutagenic effects were also observed and may be associated with the antioxidant activities detected using the CM-H(2)DCFDA probe. Conclusions: Our findings showed the following: (a) high concentrations of MB induced cytotoxicity and cell death by necrosis; (b) its antiproliferative effect was associated with G2/M arrest; and (c) its antioxidant activity could be responsible for the observed antimutagenic effects and for protective effects against gastrointestinal disorders previously described to MB. Although these effects are not specific to normal or tumor cells, they provide a panel of biological activities for further exploration. (c) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 12/01996-0 - Standardized phytoterapics for the treatment of chronic diseases: Cytotoxic, mutagenic and protective properties and the modulation of gene expression
• Grantee: Juliana Mara Serpeloni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
• Grantee: Wagner Vilegas
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants