LEEK YELLOW STRIPE VIRUS ISOLATES FROM BRAZIL FORM A DISTINCT CLADE BASED ON THE P1 GENE

Author(s)

Bampi, D. ^{[1, 2]} ; Mituti, T. ^[3] ; Pavan, M. A. ^[1] ; Hammond, J. ^[2] ; Krause-Sakate, R. ^[1]

Affiliation

^[1] UNESP, FCA, Dept Protecao Vegetal, BR-18610307 Botucatu, SP - Brazil

^[2] ARS, USDA, USNA, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 - USA

^[3] Univ Sao Paulo, ESALQ, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF PLANT PATHOLOGY; v. 97, n. 3, p. 457-463, NOV 2015.
• Web of Science Citations: 1

Abstract

The complete genome sequence of a garlic isolate of Leek yellow stripe virus (LYSV) from Brazil (LYSV-MG) was determined and its phylogenetic relationships with other LYSV isolates from other parts of the world were inferred. The LYSV-MG genome consists of 10,341 nucleotides and encodes a polyprotein of 3,221 amino acids. Brazilian LYSV isolates are more closely related to S-type than to N-type isolates but do not have a deletion in the P1 gene. Partial nucleotide sequence analysis of the P1 gene revealed 97-99% identity among Brazilian isolates, and 51-64% identity between Brazilian isolates and isolates from other countries for which sequence information is available in GenBank. The Brazilian isolates formed a monophyletic group closest to the S-type isolates and one isolate from Okinawa (O-type). These data suggest that Brazilian LYSV isolates are derived from an ancestral source of the Okinawa and S-type isolates, prior to the occurrence of the P1 deletion and divergence of the S-type isolates. (AU)

• FAPESP's process: 10/16148-9 - Survey and characterization of main Potyvirus and Carlavirus occurring in garlic, sequencing of the complete genome of Leek yellow stripe virus (LYSV) and development of techniques for detection of Shallot latent virus (SLV)
• Grantee: Marcelo Agenor Pavan
• Support Opportunities: Regular Research Grants