| Full text | |
| Author(s): |
Santos Akkari, Alessandra Cristina
[1]
;
Ramos Campos, Estefania Vangelie
[2]
;
Keppler, Artur Franz
[1]
;
Fraceto, Leonardo Fernandes
[2]
;
de Paula, Eneida
[3]
;
Tofoli, Giovana Radomille
[4]
;
de Araujo, Daniele Ribeiro
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil
[2] State Univ Julio de Mesquita Filho, Dept Enviroment Engn, Sorocaba, SP - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biochem, Campinas, SP - Brazil
[4] Fac Dent Sao Leopoldo Mand, Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | COLLOIDS AND SURFACES B-BIOINTERFACES; v. 138, p. 138-147, FEB 1 2016. |
| Web of Science Citations: | 12 |
| Abstract | |
Budesonide (BUD) is a glucocorticoid widely used for the treatment of ulcerative colitis. In this work, we propose the study of the system BUD-HP-beta-CD inclusion complex incorporated into PL 407 and PL407-PL403 thermoreversible hydrogels, considering physico-chemical and pharmaceutical aspects. Complexation between BUD and HP-beta-CD was confirmed by phase solubility studies (1:1 stoichiometry, Kc = 8662.8 M-1), DSC, FTIR and microscopy analyzes. BUD solubility in simulated upper and lower colon fluids was improved in a dependence of HP-beta-CD and PL 407 or PL407-PL403 association. Micellar hydrodynamic diameter studies showed the interaction between HP-beta-CD and PL blocks, as well as the reorganization of the micellar system in the presence of BUD and its inclusion complex. Micellization temperature (T-m) was not shifted, but sol-gel phase transition studies showed that in the presence of BUD, HP-beta-CD or BUD:HP-beta-CD complex, the association PL407-PL403 favored the gel formation close to the physiological temperature. Physico-chemical and in vitro release assays studies revealed no competitive displacement of BUD from the HP-beta-CD cavity evoked by PL407 or PL407-PL403 addition. These findings point out the BUD-HP-beta-CD in PL-based hydrogels as strategies for future investigations on development of new pharmaceutical formulations for the treatment of ulcerative colitis. (C) 2015 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 14/26200-9 - Nanostructured hybrid systems for modified release of antiinflammatpry drugs: development and pharmacological evaluation |
| Grantee: | Daniele Ribeiro de Araujo |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics |
| Grantee: | Eneida de Paula |
| Support Opportunities: | Research Projects - Thematic Grants |