Nanostructured hybrid systems for modified release of antiinflammatpry drugs: development and pharmacological evaluation

• Grant number: 14/26200-9
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2015 - July 31, 2017
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Daniele Ribeiro de Araujo
• Grantee: Daniele Ribeiro de Araujo
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated researchers: Christiane Bertachini Lombello

Abstract

The development of new carrier systems and modified release formulations have allowed the possible modulation of physical-chemical, pharmacodynamic and pharmacokinetic properties, enhancing the therapeutic effects and promoting the clinical use of different molecules. Among the various carrier systems, the self-assembled micelles, such as those formed by copolymers known as poloxamers. The poloxamers have been investigated as drug delivery systems due to their ability to form gels at temperatures close to the body (sol-gel transition), making them effective bases for inclusion complexes and nanoparticles, favoring the administration of formulations by different routes and controlling the release rate of drugs for long periods of time. The use of poloxamers with different physico-chemical characteristics (such as the poloxamers 407 and 403) is interesting to investigate properties of the hybrid systems as drugs carriers and also their influence on the sol-gel transition, micellar structure, rheological properties and the release rate of the incorporated drug. As an immediate application, the main idea of this purpose is to provide potential novel therapeutic strategies and pharmaceutical formulations for the treatment of chronic inflammatory diseases such as rheumatoid arthritis and ulcerative colitis. Thus, this proposal involves the preparation and physicochemical characterization, the in vitro cytotoxicity studies and the in vivo therapeutic efficacy after administration by intra-articular or intra-colonic routes (for naproxen and budesonide, respectively). For this reason, the drugs will be encapsulated in nanoparticles (naproxen) or complexed with cyclodextrins (budesonide) and, subsequently, dispersed in thermoreversible hydrogels, for the development of nanostructured hybrid systems. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NASCIMENTO, M. H. M.; FRANCO, M. K. K. D.; YOKAICHYIA, F.; DE PAULA, E.; LOMBELLO, C. B.; DE ARAUJO, D. R.. Hyaluronic acid in Pluronic F-127/F-108 hydrogels for postoperative pain in arthroplasties: Influence on physico-chemical properties and structural requirements for sustained drug-release. International Journal of Biological Macromolecules, v. 111, p. 1245-1254, MAY 2018. (14/14457-5, 14/26200-9, 15/14763-1)

AKKARI, ALESSANDRA C. S.; BOAVA PAPINI, JULIANA Z.; GARCIA, GABRIELLA K.; DIAS FRANCO, MARGARETH K. K.; CAVALCANTI, LEIDE P.; GASPERINI, ANTONIO; ALKSCHBIRS, MELISSA INGER; YOKAICHYIA, FABIANO; DE PAULA, ENEIDA; TOFOLI, GIOVANA R.; et al. Poloxamer 407/188 binary thermosensitive hydrogels as delivery systems for infiltrative local anesthesia: Physico-chemical characterization and pharmacological evaluation. Materials Science & Engineering C-Materials for Biological Applications, v. 68, p. 299-307, NOV 1 2016. (14/26200-9, 14/14457-5)

MONTEIRO NASCIMENTO, MONICA HELENA; SANTOS AKKARI, ALESSANDRA CRISTINA; FREITAS MARIANO, KELLY CRISTINA; KIMUS BRAZ, ANTONIO SERGIO; LOMBELLO, CHRISTIANE BERTACHINI; DE ARAUJO, DANIELE RIBEIRO. Cyclodextrin-Based Delivery Systems for Arthritic Diseases: From Development to Experimental Therapeutics. CURRENT PHARMACEUTICAL DESIGN, v. 21, n. 33, p. 4907-4916, 2015. (14/26200-9, 14/12653-1)

SANTOS AKKARI, ALESSANDRA CRISTINA; RAMOS CAMPOS, ESTEFANIA VANGELIE; KEPPLER, ARTUR FRANZ; FRACETO, LEONARDO FERNANDES; DE PAULA, ENEIDA; TOFOLI, GIOVANA RADOMILLE; DE ARAUJO, DANIELE RIBEIRO. Budesonide-hydroxypropyl-beta-cyclodextrin inclusion complex in binary poloxamer 407/403 system for ulcerative colitis treatment: A physico-chemical study from micelles to hydrogels. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 138, p. 138-147, FEB 1 2016. (14/14457-5, 14/26200-9)

FREITAS MARIANO, KELLI C.; MONTEIRO DO NASCIMENTO, MONICA H.; QUEROBINO, SAMYR M.; RAMOS CAMPOS, V, ESTEFANIA; DE OLIVEIRA, JHONES L.; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K. K. D.; ALBERTO-SILVA, CARLOS; DE PAULA, ENEIDA; LOMBELLO, CHRISTIANE B.; et al. Influence of chitosan-tripolyphosphate nanoparticles on thermosensitive polymeric hydrogels: structural organization, drug release mechanisms and cytotoxicity. INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS, v. 69, n. 9, p. 592-603, JUN 12 2020. (14/14457-5, 14/12653-1, 14/26200-9)

LAZARO, CAROLINA MARTINS; DE OLIVEIRA, CAROLINA C.; GAMBERO, ALESSANDRA; ROCHA, THALITA; SAIA CEREDA, CINTIA MARIA; DE ARAUJO, DANIELE RIBEIRO; TOFOLI, GIOVANA RADOMILLE. Evaluation of Budesonide-Hydroxypropyl-beta-Cyclodextrin Inclusion Complex in Thermoreversible Gels for Ulcerative Colitis. Digestive Diseases and Sciences, v. 65, n. 11, p. 3297-3304, NOV 2020. (14/26200-9)

Filed patent(s) as a result of this research project