Evaluation of the influence of iontophoresis on the penetration and distribution o...
| Full text | |
| Author(s): |
Lemos, Camila Nunes
[1]
;
de Souza, Joel Goncalves
[1]
;
Simao, Patricia Sper
[1]
;
Vianna Lopez, Renata Fonseca
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | PLoS One; v. 11, n. 1 JAN 11 2016. |
| Web of Science Citations: | 8 |
| Abstract | |
This study examined the potential of iontophoresis in topical photodynamic therapy (PDT) of human invasive squamous cells carcinomas (SCC). SCC was induced in nude BALB/c mice by subcutaneous injection of A431 cells. Tumor penetration and distribution of the photosensitizer tetrasulfonated zinc phthalocyanine (ZnPcS4) was investigated after 10 and 30 min of in vivo iontophoresis of a gel containing ZnPcS4. PDT was performed immediately after iontophoresis using laser at 660 nm with a dose of irradiation of 100 J/cm(2) and irradiance of 48 mW/cm(2) while tumor growth was measured for 30 days. Iontophoresis increased ZnPcS4 penetration into tumors by 6-fold after 30 min when compared with passive delivery. Confocal microscopy analysis showed that ZnPcS4 was homogeneous distributed within deep regions of the tumor after iontophoresis. Irradiation of the tumors immediately after iontophoresis showed reduction in tumor size by more than 2-fold when compared to non-treated tumors. Iontophoretic-PDT treated tumors presented large areas of necrosis. The study concluded that iontophoretic delivery of photosensitizers could be a valuable strategy for topical PDT of invasive SCC. (AU) | |
| FAPESP's process: | 11/21411-3 - Evaluation of the influence of iontophoresis on the penetration and distribution of a photosensitizing agent in hydrophilic skin cancer for topical photodynamic therapy |
| Grantee: | Camila Nunes Lemos |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue |
| Grantee: | Renata Fonseca Vianna Lopez |
| Support type: | Research Projects - Thematic Grants |