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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Baccharis dracunculifolia-based mouthrinse alters the exopolysaccharide structure in cariogenic biofilms

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Author(s):
Aires, Carolina P. [1] ; Sassaki, Guilherme L. [2] ; Santana-Filho, Arquimedes P. [2] ; Spadaro, Augusto C. C. [1] ; Cury, Jaime A. [3]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Dept Chem & Phys, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Fed Parana, Dept Biochem & Mol Biol, POB 19046, BR-81531980 Curitiba, Parana - Brazil
[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 84, p. 301-307, MAR 2016.
Web of Science Citations: 1
Abstract

Baccharis dracunculifolia is a native plant from Brazil with antimicrobial activity. The purpose of this study was to investigate whether a B. dracunculifolia-based mouthrinse (Bd) changes the structure of insoluble exopolysaccharides (IEPS) in Streptococcus mutans UA159 cariogenic biofilm. Biofilms were grown on glass slides and treated with Bd, its vehicle (VC), chlorhexidine digluconate (CHX), or saline solution (NaCl). Among the treatments, only CHX significantly reduced the biofilm biomass and bacterial viability (p < 0.05). Gas chromatography mass spectrometry and nuclear magnetic resonance analyses revealed that IEPS from the four biofilm samples were alpha-glucans containing different proportions of (1 6) and (I 3) glycosidic linkages. The structural differences among the four IEPS were compared by principal component analysis (PCA). PCA analysis indicated that IEPS from VC- and NaCl-treated biofilms were structurally similar to each other. Compared with the control, IEPS from Bd- and CHX-treated biofilms were structurally different and had distinct chemical profiles. In summary, the fact that Bd changed the IEPS chemical composition indicates that this mouthrinse may affect the cariogenic properties of the S. mutans biofilm formed. (C) 2015 Published by Elsevier B.V. (AU)

FAPESP's process: 10/12456-0 - Effect of sucrose and natural antimicrobial on chemical structure of polysaccharides from dental biofilm
Grantee:Carolina Patrícia Aires Garbellini
Support Opportunities: Regular Research Grants