Excessive eccentric exercise-induced overtraining model leads to endoplasmic reticulum stress in mice skeletal muscles

Author(s)

Pereira, Bruno C. ^[1] ; da Rocha, Alisson L. ^[1] ; Pinto, Ana P. ^[1] ; Pauli, Jose R. ^[2] ; de Souza, Claudio T. ^[3] ; Cintra, Dennys E. ^[2] ; Ropelle, Eduardo R. ^[2] ; de Freitas, Ellen C. ^[4] ; Zagatto, Alessandro M. ^[5] ; da Silva, Adelino S. R. ^{[4, 1]}

Affiliation

^[1] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Sao Paulo - Brazil

^[2] State Univ Campinas UNICAMP, Sport Sci Course, Fac Sci Appl, Sao Paulo - Brazil

^[3] Univ Far Southern Santa Catarina, Exercise Biochem & Physiol Lab Postgrad Program H, Hlth Sci Unit, Criciuma, SC - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Sao Paulo - Brazil

^[5] Univ Estadual Paulista UNESP, Lab Physiol & Sport Performance LAFIDE, Fac Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 145, p. 144-151, JAN 15 2016.
• Web of Science Citations: 14

Abstract

Aims: The present study verified the responses of selected endoplasmic reticulum (ER) stress proteins (i.e., BiP, ATF-6, pIRE1, pPERK, and peIF2alpha) in mice skeletal muscles after three different running overtraining (OT) protocols with same external load (i.e., intensity vs. volume), but performed in downhill, uphill and without inclination. Materials and methods: The rodents were randomly divided into control (CT; sedentary mice), overtrained by downhill running (OTR/down), overtrained by uphill running (OTR/up) and overtrained by running without inclination (OTR) groups. The incremental load test and exhaustive test were used as performance parameters. Forty hours after the exhaustive test performed at the end of the OT protocols (i.e., at the end of week 8) and after a 2-week total recovery period (i.e., at the end of week 10), the extensor digitorum longus (EDL) and soleus muscles were removed and used for immunoblotting. Key findings: For both skeletal muscle types, the OTR/down protocol increased the pIRE-1, pPERK and peIF2alpha, which were not normalized after the total recovery period. At the end of week 8, the other two OT protocols up-regulated the BiP, pPERK and peIF2alpha levels only for the soleus muscle. These ER stress proteins were not normalized after the total recovery period for the OTR/up group. Significance: The above findings suggest that the OTR/down protocol-induced skeletal muscle ER stress may be linked to a pathological condition in EDL and soleus muscles. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 13/20591-3 - Responses of proteins from the inflammatory, insulinic and hypertrophic molecular pathways to nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in skeletal muscle of mice
• Grantee: Adelino Sanchez Ramos da Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/19985-7 - Effects of overtraining induced by exercise in treadmill without inclination, uphill and downhill on the content and activation of proteins of insulin signaling pathway and inflamation in mice
• Grantee: Bruno Cesar Pereira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/25459-9 - Nonfunctional overreaching in animal model: inflammatory and hypertrophic adaptions of the cardiac muscle
• Grantee: Alisson Luiz da Rocha
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/08013-0 - Overreaching not functional in animal model and endoplasmic reticulum stress in muscle heart and liver
• Grantee: Ana Paula Pinto
• Support Opportunities: Scholarships in Brazil - Master