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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Burn Injury Induces Skeletal Muscle Degeneration, Inflammatory Host Response, and Oxidative Stress in Wistar Rats

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Author(s):
da Silva, Nathalia Trasmonte [1] ; Quintana, Hananiah Tardivo [1] ; Bortolin, Jeferson Andre [1] ; Ribeiro, Daniel Araki [1] ; de Oliveira, Flavia [1]
Total Authors: 5
Affiliation:
[1] Fed Univ Sao Paulo UNIFESP, Dept Biosci, Rua Silva Jardim, 136 Lab 328, BR-11015020 Santos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF BURN CARE & RESEARCH; v. 36, n. 3, p. 428-433, MAY-JUN 2015.
Web of Science Citations: 6
Abstract

Burn injuries (BIs) result in both local and systemic responses distant from the site of thermal injury, such as skeletal muscle. The purpose of this study was to investigate the expression of cyclooxygenase-2 (COX -2) and hydroxy-2'-deoxyguanosine (8-OHdG) as a result of inflammation and reactive oxygen species production, respectively. A total of 16 male rats were distributed into two groups: control (C) and submitted to BI. The medial part of gastrocnemius muscle formed the specimens, which were stained with hematoxvlin and eosin and were evaluated. COX -2 and 8-OHdG expressions were assessed by immunohistochemistry, and cell profile area and density of muscle fibers (number of fibers per square millimeter) were evaluated by morphometric methods. The results revealed inflammatory infiltrate associated with COX -2 immunoexpression in BI-gastrocnemius muscle. Furthermore, a substantial decrease in the muscle cell profile area of BI group was noticed when compared with the control group, whereas the density of muscle fibers was higher in the BI group. 8-OHdG expression in numerous skeletal muscle nuclei was detected in the BI group. In conclusion, the BI group is able to induce skeletal muscle degeneration as a result of systemic host response closely related to reactive oxygen species production and inflammatory process. (AU)

FAPESP's process: 11/22034-9 - Morphological, genotoxic and molecular effects of scalding thermal injury in gastrocnemius muscle of young rats
Grantee:Flávia de Oliveira
Support Opportunities: Regular Research Grants