Analysis of vaginal lactobacilli from healthy and infected Brazilian women

Author(s)

Artinez, Rafael C. R. M. ^[1] ; Franceschini, Silvio A. ^[2] ; Patta, Maristela C. ^[3] ; Quintana, Silvana M. ^[3] ; Nune, Alvaro C. ^[4] ; Moreira, Jodo L. S. ^[4] ; Anukarn, Kingsley C. ^[5] ; Reid, Gregor ^{[6, 7, 5]} ; De Martinis, Elaine C. P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Sistema Integrado Saude, BR-14049 Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Obstet & Ginecol, BR-14049 Ribeirao Preto - Brazil

^[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Biol Geral, Belo Horizonte, MG - Brazil

^[5] Canadian Res & Dev Ctr Probiot, Lawson Hlth Res Inst, London, ON N6A 2V4 - Canada

^[6] Univ Western Ontario, Dept Microbiol & Immunol, London, ON - Canada

^[7] Univ Western Ontario, Dept Surg, London, ON N6A 3K7 - Canada

• Document type: Journal article
• Source: Applied and Environmental Microbiology; v. 74, n. 14, p. 4539-4542, JUL 2008.
• Web of Science Citations: 23

Abstract

Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H2O2-producing lactobacilli were not associated with protection against VVC. (AU)

• FAPESP's process: 06/06595-2 - Effect of use of probiotic lactic acid bacteria on the vaginal microbiota of patients with bacterial and fungal infections
• Grantee: Elaine Cristina Pereira de Martinis
• Support Opportunities: Regular Research Grants