Detection of Schistosoma mansoni infection by TaqMan (R) Real-Time PCR in a hamster model

Author(s):Show less -

Carvalho Espirito-Santo, Maria Cristina ^{[1, 2]} ; Alvarado-Mora, Monica Viviana ^[3] ; Silva Pinto, Pedro Luiz ^[4] ; de Brito, Thales ^{[5, 1, 2]} ; Botelho-Lima, Livia ^[3] ; Heath, Ashley Richard ^[6] ; Amorim, Maria Galli ^[7] ; Dias-Neto, Emmanuel ^{[7, 8]} ; Chieffi, Pedro Paulo ^{[9, 5]} ; Rebello Pinho, Joao Renato ^[3] ; Carrilho, Flair Jose ^[3] ; Albuquerque Luna, Expedito Jose ^[5] ; Borges Gryschek, Ronaldo Cesar ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, BR-05508 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Lab Med Invest 6, BR-05508 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Dept Gastroenterol, Lab Trop Gastroenterol & Hepatol, BR-05508 Sao Paulo - Brazil

^[4] Inst Adolfo Lutz Secretaria Estado Saude Sao Paul, Dept Enteroparasites, Ctr Parasitol & Mycol, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Med Trop Sao Paulo, BR-05508 Sao Paulo - Brazil

^[6] Sigma Life Sci, The Woodlands, TX - USA

^[7] Hosp AC Camargo Fund Antonio Prudente, Ctr Int Res & Educ CIPE, Lab Med Genom, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Fac Med, Dept & Inst Psychiat, Lab Neurociencies LIM 27, BR-05508 Sao Paulo - Brazil

^[9] Fac Ciencias Med Santa Casa Sao Paulo, Sao Paulo - Brazil

• Document type: Journal article
• Source: Experimental Parasitology; v. 143, p. 83-89, AUG 2014.
• Web of Science Citations: 7

Abstract

An experimental study in hamsters was performed to evaluate the capability for detecting Schistosoma mansoni DNA in serum and fecal samples during the pre and post-egg-laying periods of infection using TaqMan (R) Real-Time PCR system (qPCR), was compared with the circumoval precipitin test (COPT) and the Kato-Katz technique, especially among individuals with low parasitic burden. Twenty-four hamsters were infected with cercariae. Three hamsters were sacrificed per week under anesthesia, from 7 days post infection (DPI) up to 56 DPI. A serum sample and a pool of feces were collected from each hamster. The presence of S. mansoni eggs in fecal samples was evaluated by Kato-Katz method and in the hamsters gut-by histopathology. Detection of S. mansoni DNA was performed using qPCR and S. mansoni antibody using COPT. The first detection of eggs in feces by Kato-Katz method and S. mansoni DNA in feces by qPCR occurred 49 DPI. Nevertheless, S. mansoni DNA was detected in serum samples from 14 up to 56 DPI. COPT was positive at 35 DPI. The results not only confirm the reliability of S. mansoni DNA detection by qPCR, but also demonstrate that serum is a trustworthy source of DNA in the pre patent infection period. (C) 2014 Elsevier Inc. All rights reserved. (AU)