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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of Candida krusei and Candida glabrata on Candida albicans gene expression in in vitro biofilms

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Author(s):
Barros, Patricia Pimentel [1] ; Ribeiro, Felipe Camargo [1] ; Rossoni, Rodnei Dennis [1] ; Junqueira, Juliana Campos [1] ; Cardoso Jorge, Antonio Olavo [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 64, p. 92-101, APR 2016.
Web of Science Citations: 13
Abstract

Objective: The present study aimed to evaluate the interactions between the species Candida albicans, Candida krusei and Candida glabrata in monotypic and mixed biofilm models formed in vitro as well as the relative expression of the ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 genes. Material and methods: Mixed (C. albicans/C. krusei and C albicans/C. glabrata) and monotypic biofilms were cultured for 0, 12 and 24 h. Gene expression was analyzed in the same biofilm model in which the number of CFU/mL was counted. Results: The C albicans CFU/mL values were lower at the 12 and 24 h time points in the mixed biofilms compared with the monotypic biofilms, and decreases of 56.23% and 64.4% in C albicans were observed when this species was associated with C glabrata and C krusei, respectively. In the presence of C krusei, the expression of the ALS3, HWP1, BCR1, EFG1 and TEC1 genes of C albicans was completely inhibited, indicating both transcriptome and the phenotypic antagonism between these two species, but genes related to the secretion of enzymes were stimulated. In the presence of C glabrata, C albicans showed a similar gene expression profile to that obtained in association with C. krusei, though it was altered to a lesser degree. Conclusion: We conclude that C. krusei and C glabrata may alter or inhibit the mechanisms involved in the in vitro adherence and formation of C albicans biofilms, influencing the pathogenicity of this species and suggesting a competitive interaction with C krusei and C glabrata in biofilm formation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/15194-0 - Interactions between different Candida species in experimental candidiasis of invertebrates and vertebrates models
Grantee:Rodnei Dennis Rossoni
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 12/15250-0 - EVALUATION OF TEMPORAL EXPRESSION OF GENES SAP5, SAP9, PLB1, PLB2, LIP9 LIP10 IN INTERACTIONS BETWEEN SPECIES C. albicans, C. tropicalis, C. glabrata and C. krusei.
Grantee:Patrícia Pimentel de Barros
Support Opportunities: Scholarships in Brazil - Doctorate