| Full text | |
| Author(s): |
Pina, Edieidia S.
[1]
;
Silva, Denise B.
[2, 3]
;
Teixeira, Simone P.
[4]
;
Coppede, Juliana S.
[1]
;
Furlan, Maysa
[5]
;
Franca, Suzelei C.
[1]
;
Lopes, Norberto P.
[2]
;
Pereira, Ana Maria S.
[1]
;
Lopes, Adriana A.
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Ribeirao Preto, Unidade Biotecnol, Av Costabile Romano 2201, BR-14096900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Fed Mato Grosso do Sul, Lab Prod Nat & Espectrometria Massas LAPNEM, BR-79070900 Campo Grande, MS - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Bot, BR-14040903 Ribeirao Preto, SP - Brazil
[5] Univ Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 6, MAR 4 2016. |
| Web of Science Citations: | 6 |
| Abstract | |
Biosynthetic investigation of quinonemethide triterpenoid 22 beta-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using C-13-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22 beta-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes. (AU) | |
| FAPESP's process: | 09/54098-6 - Emu: aquisicao de um espectrometro de massas para geracao de imagens na central de espectrometria de massas (mass-facility) da fcfrp-usp: estudos de localizacao molecular de substancias biologicamente ativas |
| Grantee: | Norberto Peporine Lopes |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 14/50265-3 - Metabolismo e distribuicao de xenobioticos naturais e sinteticos:da compreensao dos processos reacionais a geracao de imagens teciduais. |
| Grantee: | Norberto Peporine Lopes |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 12/18031-7 - Application of MALDI-MS/MS in the vicenin-2 and derivatives analyses: Understanding the ionization processes to production of the images |
| Grantee: | Denise Brentan da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/07349-9 - "Oxindole alkaloids from Uncaria tomentosa: biosynthetic studies and alkaloids analogues obtained by precursor-directed biosynthesis" |
| Grantee: | Adriana Aparecida Lopes |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/26066-3 - Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
| Grantee: | Adriana Aparecida Lopes |
| Support Opportunities: | Regular Research Grants - Publications - Scientific article |
| FAPESP's process: | 10/18132-2 - Biotechnological processes in the production of triterpenes: potential source of anticancer drug |
| Grantee: | Ana Maria Soares Pereira |
| Support Opportunities: | Regular Research Grants |