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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging

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Author(s):
Pina, Edieidia S. [1] ; Silva, Denise B. [2, 3] ; Teixeira, Simone P. [4] ; Coppede, Juliana S. [1] ; Furlan, Maysa [5] ; Franca, Suzelei C. [1] ; Lopes, Norberto P. [2] ; Pereira, Ana Maria S. [1] ; Lopes, Adriana A. [1]
Total Authors: 9
Affiliation:
[1] Univ Ribeirao Preto, Unidade Biotecnol, Av Costabile Romano 2201, BR-14096900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Fed Mato Grosso do Sul, Lab Prod Nat & Espectrometria Massas LAPNEM, BR-79070900 Campo Grande, MS - Brazil
[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Bot, BR-14040903 Ribeirao Preto, SP - Brazil
[5] Univ Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 6, MAR 4 2016.
Web of Science Citations: 6
Abstract

Biosynthetic investigation of quinonemethide triterpenoid 22 beta-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using C-13-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22 beta-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes. (AU)

FAPESP's process: 10/18132-2 - Biotechnological processes in the production of triterpenes: potential source of anticancer drug
Grantee:Ana Maria Soares Pereira
Support type: Regular Research Grants
FAPESP's process: 09/54098-6 - Acquisition of a mass spectrometer for the generation of images at the Mass Spectrometry Center of the University of São Paulo Ribeirão Preto School of Pharmaceutical Sciences: studies of molecular localization of biologically active substances
Grantee:Norberto Peporine Lopes
Support type: Multi-user Equipment Program
FAPESP's process: 14/50265-3 - Distribution and metabolism of natural and synthetic xenobiotics: from the comprehension of reactional process to tissue imaging generation
Grantee:Norberto Peporine Lopes
Support type: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 15/26066-3 - Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging
Grantee:Adriana Aparecida Lopes
Support type: Regular Research Grants - Publications - Scientific article
FAPESP's process: 13/07349-9 - Oxindole alkaloids from Uncaria tomentosa: biosynthetic studies and alkaloids analogues obtained by precursor-directed biosynthesis
Grantee:Adriana Aparecida Lopes
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/18031-7 - Application of MALDI-MS/MS in the vicenin-2 and derivatives analyses: Understanding the ionization processes to production of the images
Grantee:Denise Brentan da Silva
Support type: Scholarships in Brazil - Post-Doctorate