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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status

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Author(s):
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dos Santos Hamilton, Thais Rose [1] ; de Castro, Leticia Signori [1] ; Delgado, Juliana de Carvalho [1] ; de Assis, Patricia Monken [1] ; Perez Siqueira, Adriano Felipe [1] ; Mendes, Camilla Mota [1] ; Goissis, Marcelo Demarchi [1] ; Muino-Blanco, Teresa [2] ; Cebrian-Perez, Jose Alvaro [2] ; Nichi, Marcilio [1] ; Visintin, Jose Antonio [1] ; Ortiz D'Avila Assumpcao, Mayra Elena [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Ave Prof Dr Orlando Marques Paiva 87, Cidade Univ, BR-05508270 Sao Paulo - Brazil
[2] Univ Zaragoza, Dept Biochem & Mol & Cell Biol, Inst Invest Ciencias Ambientales Aragon IUCA, Grp Biol & Fisiol Reprod, Fac Vet, Miguel Servet 177, Zaragoza 50013 - Spain
Total Affiliations: 2
Document type: Journal article
Source: Reproduction; v. 151, n. 4, p. 379-390, APR 2016.
Web of Science Citations: 14
Abstract

Action of reactive oxygen species, protamination failures and apoptosis are considered the most important etiologies of sperm DNA fragmentation. This study evaluated the effects of induced lipid peroxidation susceptibility on native semen profile and identified the mechanisms involved in sperm DNA fragmentation and testicular antioxidant defense on Santa Ines ram sperm samples. Semen was collected from 12 adult rams (Ovis aries) performed weekly over a 9-week period. Sperm analysis (motility, mass motility, abnormalities, membrane and acrosome status, mitochondrial potential, DNA fragmentation, lipid peroxidation and intracellular free radicals production); protamine deficiency; PRM1, TNP1 and TNP2 gene expression; and determination of glutathione peroxidase (GPx), glutathione reductase, catalase (CAT) and superoxide dismutase activity and immunodetection in seminal plasma were performed. Samples were distributed into four groups according to the sperm susceptibility to lipid peroxidation after induction with ascorbate and ferrous sulfate (low, medium, high and very high). The results were analyzed by GLM test and post hoc least significant difference. We observed an increase in native GPx activity and CAT immunodetection in groups with high susceptibility to induced lipid peroxidation. We also found an increase in total sperm defects, acrosome and membrane damages in the group with the highest susceptibility to induced lipid peroxidation. Additionally, the low mitochondrial membrane potential, susceptible to chromatin fragmentation and the PRM1 mRNA were increased in the group showing higher susceptibility to lipid peroxidation. Ram sperm susceptibility to lipid peroxidation may compromise sperm quality and interfere with the oxidative homeostasis by oxidative stress, which may be the main cause of chromatin damage in ram sperm. (AU)

FAPESP's process: 13/05025-1 - Measurement of antioxidant enzymes in the ram's semen: cellular and plasma concentrations
Grantee:Thais Rose dos Santos Hamilton
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 11/11231-8 - Study of factors involved in DNA fragmentation of spermatozoa in ram.
Grantee:Thais Rose dos Santos Hamilton
Support Opportunities: Scholarships in Brazil - Doctorate