Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Full text
Author(s):
Cardoso, J. C. [1, 2, 3] ; Abdelgalel, A. M. [4] ; Chiancone, B. [4] ; Latado, R. R. [3] ; Lain, O. [5] ; Testolin, R. [5] ; Germana, M. A. [4]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Ctr Energia Nucl Agr, Piracicaba, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Desenvolvimento Rural, Ctr Ciencias Agr, Rod Anhanguera, Km 174, CP 153, BR-13600970 Araras, SP - Brazil
[3] Inst Agron, Ctr APTA Citros Sylvio Moreira, Cordeiropolis, SP - Brazil
[4] Univ Palermo, Dipartimento Sci Agr & Forestali, Viale Sci 11, I-90128 Palermo - Italy
[5] Dipartimento Sci Agr & Ambientali, Viale Sci 208, I-33100 Udine - Italy
Total Affiliations: 5
Document type: Journal article
Source: PLANT BIOSYSTEMS; v. 150, n. 2, p. 304-312, MAR 3 2016.
Web of Science Citations: 3
Abstract

In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos). (AU)

FAPESP's process: 08/10203-8 - Anther culture and in situ parthenogenesis of sweet orange cultivars (Citrus sinensis Osbeck.)
Grantee:Rodrigo Rocha Latado
Support Opportunities: Regular Research Grants
FAPESP's process: 08/09928-8 - Anther culture and in situ parthenogenesis of sweet orange (Citrus sinensis Osbeck.) cultivars
Grantee:Jean Carlos Cardoso
Support Opportunities: Scholarships in Brazil - Doctorate