| Full text | |
| Author(s): |
Priscila Preve Pereira
[1]
;
Anelise Maria Bosco
[2]
;
Breno Fernando Martins de Almeida
[3]
;
Luis Gustavo Narciso
[4]
;
Paulo César Ciarlini
[5]
Total Authors: 5
|
| Affiliation: | [1] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias - Brasil
[2] Universidade Estadual Paulista. Faculdade de Medicina Veterinária de Araçatuba - Brasil
[3] Universidade Estadual Paulista. Faculdade de Medicina Veterinária de Araçatuba - Brasil
[4] Universidade Estadual Paulista. Faculdade de Medicina Veterinária de Araçatuba - Brasil
[5] Universidade Estadual Paulista. Faculdade de Medicina Veterinária de Araçatuba - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Ciênc. anim. bras.; v. 16, n. 4, p. 560-566, 2015-12-00. |
| Abstract | |
Abstract Among the uremic toxins proven to affect the neutrophil function in humans with chronic kidney disease (CKD), guanidine compounds stand out. To achieve a clearer understanding of the mechanisms that affect the immunity of uremic patients, the hypothesis that guanidine acetic acid (GAA) contributes to the inhibition of oxidative metabolism and an increase in neutrophil apoptosis in healthy dogs was investigated in vitro. To this end, neutrophils isolated from ten healthy dogs were incubated in pure RPMI 1640 (control) and enriched with 5 mg/L of GAA. Capillary flow cytometry was used to quantify superoxide production in neutrophils with the probe (hydroethidine), in the presence and absence of phorbol-12-myristate-13-acetate (PMA), in order to assess oxidative metabolism. Apoptotic indices were quantified using the Annexin V-PE system, with and without the inductive effect of camptothecin. Neutrophils isolated and incubated in a GAA-enriched medium produced smaller amounts of superoxide (p<0.001) when activated with PMA, however, this inhibition of oxidative metabolism occurred without significantly altering their viability or rate of apoptosis. Thus, the results show guanidine compounds contribute to immunosuppression in dogs with CKD. (AU) | |
| FAPESP's process: | 11/18746-3 - Effect of p-cresol and guanidine on the oxidative metabolism and apoptosis of neutrophils of dogs |
| Grantee: | Paulo César Ciarlini |
| Support Opportunities: | Regular Research Grants |