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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of adipose tissue-derived stem cells in the progression of renal disease

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Author(s):
Cassiano Donizetti-Oliveira ; Patricia Semedo ; Marina Burgos-Silva ; Marco Antonio Cenedeze ; Denise Maria Avancini Costa Malheiros ; Marlene Antônia dos Reis ; Alvaro Pacheco-Silva ; Niels Olsen Saraiva Câmara
Total Authors: 8
Document type: Journal article
Source: Einstein (São Paulo); v. 9, n. 1, p. 36-45, Mar. 2011.
Abstract

ABSTRACTObjective:To analyze the role of adipose tissue-derived stem cells in reducing the progression of renal fibrosis.Methods:adipose tissue-derived stem cells were isolated from C57Bl/6 mice and characterized by cytometry and differentiation. Renal fibrosis was established after unilateral clamping of the renal pedicle for 1 hour. Four hours after reperfusion, 2.105 adipose tissue-derived stem cells were administered intraperitoneally and the animals were followed for 24 hours during 6 weeks. In another experimental group, 2.105adipose tissue-derived stem cells were administered only after 6 weeks of reperfusion, and they were euthanized and studied 4 weeks later. Twenty-four hours after reperfusion, the animals treated with adipose tissue-derived stem cells displayed reduced renal and tubular dysfunction and an increase of the regenerative process. Renal expression of IL-6 and TNF mRNA were decreased in the animals treated with adipose tissue-derived stem cells, while the levels of IL-4, IL-10, and HO-1 were increased, despite the fact that adipose tissue-derived stem cells were not observed in the kidneys via SRY analysis.Results:In 6 weeks, the kidneys of non-treated animals decreased in size, and the kidneys of the animals treated with adipose tissue-derived stem cells remained at normal size and display less deposition of type 1 collagen and FSP-1. The renal protection observed in animals treated with adipose tissue-derived stem cells was followed by a drop in serum levels of TNF-α, KC, RANTES, and IL-1a. Treatment with adipose tissue-derived stem cells after 6 weeks, when the animals already displayed established fibrosis, demonstrated an improvement in functional parameters and less fibrosis analyzed by Picrosirius stain, as well as a reduction of the expression of type 1 collagen and vimentin mRNA.Conclusion:Treatment with adipose tissue-derived stem cells may deter the progression of renal fibrosis by modulation of the early inflammatory response, likely via reduction of the epithelial-mesenchymal transition. (AU)

FAPESP's process: 07/07139-3 - The role of heme oxygenase 1 in different renal inflammatory process in experimental animal models
Grantee:Niels Olsen Saraiva Câmara
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/13251-6 - Role of stem cells derived from adipose tissue in the progression of renal disease
Grantee:Cassiano Donizetti de Oliveira
Support type: Scholarships in Brazil - Master