| Full text | |
| Author(s): |
Cassiano Donizetti-Oliveira
[1]
;
Patricia Semedo
[2]
;
Marina Burgos-Silva
[3]
;
Marco Antonio Cenedeze
[4]
;
Denise Maria Avancini Costa Malheiros
[5]
;
Marlene Antônia dos Reis
[6]
;
Alvaro Pacheco-Silva
[7]
;
Niels Olsen Saraiva Câmara
[8]
Total Authors: 8
|
| Affiliation: | [1] Universidade Federal de São Paulo – UNIFESP. Department of Medicine, Escola Paulista de Medicina. Clinical and Experimental Immunology Laboratory of the Discipline of Nephrology - Brasil
[2] Universidade Federal de São Paulo – UNIFESP. Department of Medicine, Escola Paulista de Medicina. Clinical and Experimental Immunology Laboratory of the Discipline of Nephrology - Brasil
[3] Universidade Federal de São Paulo – UNIFESP. Department of Medicine, Escola Paulista de Medicina. Clinical and Experimental Immunology Laboratory of the Discipline of Nephrology - Brasil
[4] Universidade Federal de São Paulo – UNIFESP. Department of Medicine, Escola Paulista de Medicina. Clinical and Experimental Immunology Laboratory of the Discipline of Nephrology - Brasil
[5] Universidade de São Paulo – USP. Faculty of Medicine. Department of Pathology - Brasil
[6] Universidade Federal do Triângulo Mineiro – UFTM. Faculty of Medicine. Discipline of Pathology - Brasil
[7] Universidade Federal de São Paulo – UNIFESP. Department of Medicine, Escola Paulista de Medicina. Clinical and Experimental Immunology Laboratory of the Discipline of Nephrology - Brasil
[8] Universidade Federal de São Paulo – UNIFESP. Department of Medicine, Escola Paulista de Medicina. Clinical and Experimental Immunology Laboratory of the Discipline of Nephrology - Brasil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | Einstein (São Paulo); v. 9, n. 1, p. 36-45, 2011-03-00. |
| Abstract | |
ABSTRACT Objective: To analyze the role of adipose tissue-derived stem cells in reducing the progression of renal fibrosis. Methods: adipose tissue-derived stem cells were isolated from C57Bl/6 mice and characterized by cytometry and differentiation. Renal fibrosis was established after unilateral clamping of the renal pedicle for 1 hour. Four hours after reperfusion, 2.105 adipose tissue-derived stem cells were administered intraperitoneally and the animals were followed for 24 hours during 6 weeks. In another experimental group, 2.105 adipose tissue-derived stem cells were administered only after 6 weeks of reperfusion, and they were euthanized and studied 4 weeks later. Twenty-four hours after reperfusion, the animals treated with adipose tissue-derived stem cells displayed reduced renal and tubular dysfunction and an increase of the regenerative process. Renal expression of IL-6 and TNF mRNA were decreased in the animals treated with adipose tissue-derived stem cells, while the levels of IL-4, IL-10, and HO-1 were increased, despite the fact that adipose tissue-derived stem cells were not observed in the kidneys via SRY analysis. Results: In 6 weeks, the kidneys of non-treated animals decreased in size, and the kidneys of the animals treated with adipose tissue-derived stem cells remained at normal size and display less deposition of type 1 collagen and FSP-1. The renal protection observed in animals treated with adipose tissue-derived stem cells was followed by a drop in serum levels of TNF-α, KC, RANTES, and IL-1a. Treatment with adipose tissue-derived stem cells after 6 weeks, when the animals already displayed established fibrosis, demonstrated an improvement in functional parameters and less fibrosis analyzed by Picrosirius stain, as well as a reduction of the expression of type 1 collagen and vimentin mRNA. Conclusion: Treatment with adipose tissue-derived stem cells may deter the progression of renal fibrosis by modulation of the early inflammatory response, likely via reduction of the epithelial-mesenchymal transition. (AU) | |
| FAPESP's process: | 07/07139-3 - The role of heme oxygenase 1 in different renal inflammatory process in experimental animal models |
| Grantee: | Niels Olsen Saraiva Câmara |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 09/13251-6 - Role of stem cells derived from adipose tissue in the progression of renal disease |
| Grantee: | Cassiano Donizetti de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Master |