Transfusion-Transmitted Dengue and Associated Clinical Symptoms During the 2012 Epidemic in Brazil

Author(s):Show less -

Sabino, Ester C. ^[1] ; Loureiro, Paula ^[2] ; Lopes, Maria Esther ^[3] ; Capuani, Ligia ^[1] ; McClure, Christopher ^[4] ; Chowdhury, Dhuly ^[4] ; Di-Lorenzo-Oliveira, Claudia ^[5] ; Oliveira, Lea C. ^[1] ; Linnen, Jeffrey M. ^[6] ; Lee, Tzong-Hae ^[7] ; Goncalez, Thelma ^[7] ; Brambilla, Donald ^[4] ; Kleinman, Steve ^{[7, 8]} ; Busch, Michael P. ^{[9, 7]} ; Custer, Brian ^{[9, 7]} ; Epid, Int Component NHLBI Recipient

Affiliation

^[1] Univ Sao Paulo, Inst Trop Med, Dep Molestias Infecciosas & Parasitarias, Recife, PE - Brazil

^[2] UPE Fundacao Hemope, Fac Ciencias Med, Recife, PE - Brazil

^[3] Fundacao Hemorio, Rio De Janeiro - Brazil

^[4] RTI Int, Rockville, MD - USA

^[5] Univ Fed Sao Joao del Rey, Divinopolis - Brazil

^[6] Hologic, San Diego, CA - USA

^[7] Blood Syst Res Inst, 270 Masonic Ave, San Francisco, CA 94118 - USA

^[8] Univ British Columbia, Vancouver, BC V5Z 1M9 - Canada

^[9] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 - USA

• Document type: Journal article
• Source: Journal of Infectious Diseases; v. 213, n. 5, p. 694-702, MAR 1 2016.
• Web of Science Citations: 41

Abstract

Background. A linked donor-recipient study was conducted during epidemics in 2 cities in Brazil to investigate transfusion-transmitted (TT) dengue virus (DENV) by DENV RNA-positive donations. Methods. During February-June 2012, samples were collected from donors and recipients and retrospectively tested for DENV RNA by transcription-mediated amplification. Recipient chart review, using a case (DENV positive)-control (DENV negative and not known to be exposed) design, was conducted to assess symptoms. Results. Of 39 134 recruited blood donors, DENV-4 viremia was confirmed in 0.51% of donations from subjects in Rio de Janeiro and 0.80% of subjects in Recife. Overall, 42 DENV RNA-positive units were transfused into 35 recipients. Of these, 16 RNA-positive units transfused into 16 susceptible recipients were identified as informative: 5 cases were considered probable TT cases, 1 possible TT case, and 10 nontransmissions. The TT rate was 37.5% (95% confidence interval {[}CI], 15.2%-64.6%), significantly higher than the viremia rate of 0.93% (95% CI, .11%-3.34%) in nonexposed recipients (P < .0001). Chart review did not find significant differences between cases and controls in symptoms or mortality. Conclusions. During a large epidemic of DENV-4 infection in Brazil, >0.5% of donations were RNA positive, and approximately one third of components resulted in TT. However, no significant clinical differences were evident between RNA-positive and RNA-negative recipients. (AU)

• FAPESP's process: 11/18955-1 - Dengue prevalence in blood donors and recipients in Brazil: index of transmission by transfusion and clinical outcomes of dengue infection in recipients
• Grantee: Ester Cerdeira Sabino
• Support Opportunities: Regular Research Grants