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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants

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Author(s):
Vicente, Filipa A. [1] ; Lario, Luciana D. [2] ; Pessoa, Jr., Adalberto [2] ; Ventura, Sonia P. M. [1]
Total Authors: 4
Affiliation:
[1] Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro - Portugal
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Process Biochemistry; v. 51, n. 4, p. 528-534, APR 2016.
Web of Science Citations: 11
Abstract

Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/23726-4 - "purification of proteases secreted by the psicrotroph yeast Rhodoturola mucilagionosa by aqueous two-phase systems (PEG/NaPA) and chromatographic processes"
Grantee:Luciana Daniela Lario
Support type: Scholarships in Brazil - Post-Doctorate