Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants

Author(s)

Vicente, Filipa A. ^[1] ; Lario, Luciana D. ^[2] ; Pessoa, Jr., Adalberto ^[2] ; Ventura, Sonia P. M. ^[1]

Affiliation

^[1] Univ Aveiro, Dept Quim, CICECO, P-3810193 Aveiro - Portugal

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo - Brazil

• Document type: Journal article
• Source: Process Biochemistry; v. 51, n. 4, p. 528-534, APR 2016.
• Web of Science Citations: 11

Abstract

Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 12/23726-4 - "purification of proteases secreted by the psicrotroph yeast Rhodoturola mucilagionosa by aqueous two-phase systems (PEG/NaPA) and chromatographic processes"
• Grantee: Luciana Daniela Lario
• Support Opportunities: Scholarships in Brazil - Post-Doctoral