Molecular characterization of Capim and Enseada orthobunyaviruses

Author(s)

de Souza, William Marciel ^[1] ; Acrani, Gustavo Olszanski ^[2] ; Romeiro, Marilia Farignoli ^[1] ; Reis, Jr., Osvaldo ^[3] ; Tolardo, Aline Lavado ^[1] ; da Silva, Sandro Patroca ^[4] ; de Almeida Medeiros, Daniele Barbosa ^[5] ; Varela, Mariana ^[6] ; Teixeira Nunes, Marcio Roberto ^[4] ; Moraes Figueiredo, Luiz Tadeu ^[1]

Affiliation

^[1] Univ Sao Paulo, Virol Res Ctr, Sch Med Ribeirao Preto, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Dept Cell & Mol Biol, Sch Med, BR-14049 Ribeirao Preto - Brazil

^[3] Univ Estadual Campinas UNICAMP, Lab Cent Tecnol Alto Desempenho Ciencias Vida LaC, Campinas - Brazil

^[4] Inst Evandro Chagas, Ctr Technol Innovat, Ananindeua - Brazil

^[5] Inst Evandro Chagas, Dept Arbovirol & Febres Hemorrag, Ananindeua - Brazil

^[6] Univ Glasgow, MRC, Ctr Virus Res, 464 Bearsden Rd, Glasgow G61 1QH, Lanark - Scotland

• Document type: Journal article
• Source: INFECTION GENETICS AND EVOLUTION; v. 40, p. 47-53, JUN 2016.
• Web of Science Citations: 8

Abstract

Capim and Enseada viruses are members of the genus Orthobunyavirus isolated from mosquitoes and mammals in Brazil. Despite seroprevalence studies indicating human infections in Latin America, these viruses remain relatively unknown and unstudied. In order to better understand the genetic and evolutionary relationships among orthobunyaviruses, we sequenced the three genomic segments of Capim and Enseada orthobunyaviruses. Based on phylogenetic analysis, we demonstrated that these viruses depicted two new distinct clades, one represented by Enseada and another composed of Capim virus. In general, the genome organization and genetic traits of these viruses are similar to other orthobunyaviruses however, the open reading frame (ORF) of the putative nonstructural NSs protein of Enseada orthobunyavirus precedes the nucleocapsid ORF. Overall, our study provides details on the molecular characteristics of the prototype species of two groups within the Orthobunyavirus genus, revealing novel features into the genetic diversity and evolution of this genus. (c) 2016 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/20851-8 - Study about Alphavirus and Flavivirus infections in samples of blood bank
• Grantee: Marilia Farignoli Romeiro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/05778-5 - Bioinformatics analysis of data of viral metagenomic of animals from Brazil and Antarctica
• Grantee: William Marciel de Souza
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 13/14929-1 - Detection of arbovirus and other RNA viruses by real time RT-PCR and study of the viroma
• Grantee: Luiz Tadeu Moraes Figueiredo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/02438-6 - Studies with Bunyaviridae that produce human disease
• Grantee: Luiz Tadeu Moraes Figueiredo
• Support Opportunities: Research Projects - Thematic Grants