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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Contrasting strategies used by lichen microalgae to cope with desiccation-rehydration stress revealed by metabolite profiling and cell wall analysis

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Author(s):
Centeno, Danilo C. [1, 2] ; Hell, Aline F. [2] ; Braga, Marcia R. [2] ; del Campo, Eva M. [3] ; Casano, Leonardo M. [3]
Total Authors: 5
Affiliation:
[1] Fed Univ ABC, Ctr Nat Sci & Humanities, BR-09606070 Sao Bernardo Do Campo, SP - Brazil
[2] Inst Bot, Dept Plant Physiol & Biochem, BR-04301912 Sao Paulo, SP - Brazil
[3] Univ Alcala de Henares, Dept Life Sci, Alcala De Henares 28805, Madrid - Spain
Total Affiliations: 3
Document type: Journal article
Source: ENVIRONMENTAL MICROBIOLOGY; v. 18, n. 5, p. 1546-1560, MAY 2016.
Web of Science Citations: 9
Abstract

Most lichens in general, and their phycobionts in particular, are desiccation tolerant, but their mechanisms of desiccation tolerance (DT) remain obscure. The physiological responses and cell wall features of two putatively contrasting lichen-forming microalgae, Trebouxia sp. TR9 (TR9), isolated from Ramalina farinacea (adapted to frequent desiccation-rehydration cycles), and Coccomyxa solorina-saccatae (Csol), obtained from Solorina saccata (growing in usually humid limestone crevices, subjected to seasonal dry periods) was characterized. Microalgal cultures were desiccated under 25%-30% RH and then rehydrated. Under these conditions, RWC and psi(w) decreased faster and simultaneously during dehydration in Csol, whereas TR9 maintained its psi(w) until 70% RWC. The metabolic profile indicated that polyols played a key role in DT of both microalgae. However, TR9 constitutively accumulated higher amounts of polyols, whereas Csol induced the polyol synthesis under desiccation-rehydration. Csol also accumulated ascorbic acid, while TR9 synthesized protective raffinose-family oligosaccharides (RFOs) and increased its content of phenolics. Additionally, TR9 exhibited thicker and qualitatively different cell wall and extracellular polymeric layer compared with Csol, indicating higher water retention capability. The findings were consistent with the notion that lichen microalgae would have evolved distinct strategies to cope with desiccation-rehydration stress in correspondence with the water regime of their respective habitats. (AU)

FAPESP's process: 12/16332-0 - Storage and cell wall carbohydrates from plants and filamentous fungi: changes in response to alelochemicals and environmental conditions
Grantee:Marcia Regina Braga
Support type: Regular Research Grants
FAPESP's process: 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers
Grantee:Marcia Regina Braga
Support type: Research Projects - Thematic Grants